宽边黄粉蝶(Eurema hecabe)基因组Survey分析

Genome Survey Analysis in the Common Grass Yellow Butterfly(Eurema hecabe)

宽边黄粉蝶(Eurema hecabe)是重要的传粉昆虫,广布于非洲热带区、东洋区、澳洲区及古北区东部,具有较高的学术价值和经济意义。为了深入研究其广泛适应性,确定适合该蝴蝶的全基因组的测序研究策略,我们首先做低覆盖度的基因组Survey测序,然后做大规模的全基因组深度测序。采用第二代高通量的测序技术作为该研究的研究方法,测定了宽边黄粉蝶基因组大小,并利用生物信息学方法估计该种的杂合率、重复序列和GC含量等基因组信息。结果表明:(1)宽边黄粉蝶的基因组大小估计为285.34 Mb,测序深度51×;(2)从K-mer分布曲线发现黄粉蝶基因组有明显的杂合峰,杂合率达1.97%,重复序列比例为35.37%。该研究结果对于揭示宽边黄粉蝶物种的起源和进化及适应性具有重要意义,为宽边黄粉蝶选择全基因组测序策略提供依据。

The common grass yellow butterfly(Eurema hecabe) is an important pollinating insect widely distributed in the tropical regions of Africa, East Asia, Australia, and the eastern part of the Palaearctic region. It has high academic value and economic significances. In order to thoroughly study its wide applicability, prior to large-scale genome-wide deep sequencing, genome survey sequencing with low coverage was performed to evaluate the size and complexity of the genome to determine sequencing strategies that were suitable for the whole genome of the butterfly. In this study, the second-generation high-throughput sequencing technology was first used to determine the size of this butterfly, and bioinformatics methods were used to estimate the genomic information such as heterozygosity, repeat sequence, and GC content. The results showed that:(1) the genome size of the common grass yellow butterfly was estimated to be about 285.34 Mb, and the sequencing depth was 51×;(2) the heterozygous peak was observed from the K-mer distribution curve and the heterozygous rate was 1.97%. The proportion of repeated sequences was 35.37%. The results of this study have important significance for revealing the origin, evolution and adaptability of the butterfly species, and provide the basis for the selection of whole genome sequencing strategies.