菹草提取物总黄酮、总酚酸和抗氧化活性含量研究

Study on the Content of Total Flavonoids, Total Phenolic Acid and Antioxidant Activity of Extracts from Potamogeton crispus L.

研究菹草提取物的有效成分,测定其总黄酮含量及总酚酸含量,并确定它的乙酸乙酯和石油醚部位的抗氧化活性成分。采用紫外可见分光光度法,以芦丁和没食子酸作为控制材料,测定菹草的石油醚部位及乙酸乙酯部位中总黄酮和总酚酸含量;采用清除DPPH自由基能力测定法、测定总还原能力铁氰化钾法和水杨酸捕捉羟基自由基法来对菹草各组分提取物的抗氧化能力进行研究。菹草中有一定量的黄酮类化合物和酚酸类化合物存在,不同溶剂提取的部位所含有的总黄酮和总酚酸含量是有差异的,石油醚部位的总黄酮含量要高于乙酸乙酯部位的总黄酮含量,并且总酚酸的含量亦是如此;石油醚部位和乙酸乙酯部位的提取物具有清除DPPH、羟基自由基和还原Fe^3+的能力,各部位清除DPPH、羟基自由基的能力和还原Fe^3+的能力随着样品浓度的增大而增大,且乙酸乙酯部位的测定结果均低于石油醚部位。

In this paper, the active components of the extract were researched, the content of total flavonoids and total phenolic acid was tested, and its antioxidant activity too. The content of total flavonoids and total phenolic acids in petroleum ether and ethyl acetate were tested by ultraviolet spectrophotometry using rutin and gallic acid as control materials. The antioxidant activity of extracts from different solvent of Potamogeton crispus L. was tested by DPPH free radical scavenging, Potassium ferricyanide reduction and salicylic acid capturing hydroxyl radical method. There is a certain amount of total flavonoids and total phenolic acid in it. The content of them in different solvent extract was different, and the total flavonoids content of the petroleum ether extract was higher than the ethyl acetate extract, and the total phenolic acid content was the same. The extract from petroleum ether and ethyl acetate has the ability to scavenge DPPH, hydroxyl radicals and to restore Fe^3+. The ability of each extract enhanced with the increasing of sample concentration, and the results of ethyl acetate extract was lower than petroleum ether one.