肾细胞癌中自然杀伤细胞的可塑性及其临床作用的研究

Plasticity of natural killer cells and its clinical role in renal cell carcinoma

目的检测自然杀伤细胞(NK细胞)在肾细胞癌(RCC)患者癌组织和癌旁组织中可塑性及其与疾病的相关性。方法收集30例RCC患者的癌组织和癌旁组织,经过密度梯度离心法获得组织浸润的淋巴细胞,采用流式细胞术检测组织浸润的淋巴细胞中NK细胞比例,统计其表型分布的特征,并分析其变化与肿瘤分期之间的相关性。结果与癌旁组织(8.53±3.00)%相比,癌组织中NK细胞的比例明显增加(14.43±3.96)%;且与癌旁组织比,癌组织中NK细胞高表达1型固有淋巴细胞(ILC1)的表面标志CD127[(7.97±2.65)%vs.(12.66±2.88)%]、CD49a[(8.70±3.90)%vs.(16.49±5.02)%]、TRAIL[(13.77±3.82)%vs.(19.76±3.75)%],低表达NK细胞的转录因子Eomes[(9.20±3.41)%vs.(4.82±1.20)%]。同时ILC1样细胞的比例随着肿瘤TNM分期增高逐渐增多。结论RCC患者癌组织中NK细胞转换为ILC1样细胞,且与疾病进展密切相关。

Objective To detect the plasticity of natural killer cells(NK cells)in tumor tissues and adjacent tissues of patients with renal cell carcinoma(RCC)and their correlation with disease.Methods The tumor tissues and adjacent tissues of 30 patients with RCC were collected.The infiltrating lymphocytes were obtained by density gradient centrifugation,and the proportion of NK cells in infiltrating lymphocytes was detected by flow cytometry.The characteristics of their phenotypic distribution were analyzed,and the correlation between the changes and tumor stage was analyzed.Results Compared with the adjacent tissues[(8.53±3.00)%],the proportion of NK cells in tumor tissues[(14.43±3.96)%]increased significantly.Compared with the adjacent tissues,intrinsic lymphocyte cell type 1(ILC1)related surface markers,including CD127[(7.97±2.65)%vs.(12.66±2.88)%],CD49 a[(8.70±3.90)%vs.(16.49±5.02)%],and TRAIL[(13.77±3.82)%vs.(19.76±3.75)%]increased in NK cells of tumor tissues,and Eomes[(9.20±3.41)%vs.(4.82±1.20)%],a transcription factor of NK cells,decreased in NK cells of tumor tissues.The proportion of ILC1-like cells gradually increased with the progress of TNM stage.Conclusion NK cells in tumor tissues transformed into ILC1-like cells in patients with RCC,and these ILC1-like cells were closely related to disease progress.