重金属耦合剂(乙二胺四乙酸和柠檬酸钠)与抗氧化剂(硫辛酸和乙酰肉碱)对小鼠卵子数目、线粒体的影响

Effects of Heavy Metal Coupling Agents(Ethylenediaminetetraacetic Acid and Sodium Citrate) and Antioxidants(Lipoic Acid and Acetylcarnitine) on the Number of Eggs and Mitochondria in Mice

目的:探讨分析小鼠卵子数目和线粒体活性受重金属耦合剂(乙二胺四乙酸和柠檬酸钠)与抗氧化剂(硫辛酸和乙酰肉碱)的影响。方法:研究对象选定为148只G57BL/6雌性小鼠,因在饲养期间发生互咬等正常死亡,最终参加实验的小鼠共144只。依据随机分组方式将144只小鼠为四组,每组各36只。使用重金属耦合剂(乙二胺四乙酸和柠檬酸钠)的小鼠为重金属组,使用抗氧化剂(硫辛酸和乙酰肉碱)的小鼠为抗氧化组,使用重金属耦合剂与抗氧化剂小鼠为混合组,未使用两种试剂的小鼠为对照组。雌性小鼠饲养3、6、9、12个月后,对比分析四组研究对象的线粒体和卵子数目的各项临床指标。结果:四组研究小鼠饲养3、6个月线粒体活性比较,差异均无统计学意义(P>0.05)。四组研究小鼠的卵子在3、6个月的线粒体活性均明显高于9个月,差异均有统计学意义(P<0.05)。抗氧化组与混合组在9个月后的线粒体活性均明显高于重金属组与对照组,差异均有统计学意义(P<0.05)。对照组在3个月时的卵子数目明显均低于其他三组,差异均有统计学意义(P<0.05)。抗氧化组与混合组在6、9个月的卵子数目均明显高于重金属组与对照组,差异均有统计学意义(P<0.05)。抗氧化组与混合组、重金属组与对照组在6、9、12个月时卵子数目比较,差异均无统计学意义(P>0.05)。结论:随年龄增长,小鼠卵子的线粒体活性降低,卵子数目减少。小鼠摄入适量抗氧化剂硫辛酸和乙酰肉碱,可延缓卵母细胞老化。对人类的抗卵子老化有一定的参考价值。

Objective: To investigate the effects of heavy metal couplants(Ethylenediaminetetraacetic Acid and Sodium Citrate) and Antioxidants(Lipoic Acid and Acetylcarnitine) on the number of eggs and mitochondrial activity in mice. Method: A total of 148 female mice of G57 BL/6 were selected for the study. 144 mice were finally involved in the experiment due to normal death such as biting each other during the feeding period. 144 mice were randomly divided into 4 groups, 36 mice in each group, the heavy metal couplants group(Ethylenediaminetetraacetic Acid and Sodium Citrate), the antioxidants group(Lipoic Acid and Acetylcarnitine), the heavy metal couplants and antioxidants group, that did not use the two reagents in the experiment was the control group. After 3, 6, 9 and 12 months, the clinical indexes of mitochondria and egg number of four groups were compared and analyzed. Result: There were no significant differences in mitochondrial activity among the four groups after 3 and 6 months(P>0.05). After 3 and 6 months, the activity of mitochondria in the eggs of the four groups were significantly higher than those of 9 months(P<0.05). After 9 months, the activity of mitochondria in antioxidant group and mixed group were significantly higher than those in heavy metal group and control group(P<0.05). The number of eggs in the control group were significantly lower than those in the other three groups at 3 months(P<0.05). The number of eggs at 6 and 9 months in antioxidant group and mixed group were significantly higher than those of heavy metal group and control group(P<0.05). There were no significant differences in the number of eggs between antioxidant group and mixed group, heavy metal group and control group at 6, 9 and 12 months(P>0.05). Conclusion: With the increase of age, the mitochondrial activity and the number of eggs decreased. The aging of oocytes could be delayed by appropriate intake of lipoic acid and acetylcarnitine. It has a certain reference value for human anti egg aging.