建立雾霾伤肺温燥证动物模型的初步探讨

Preliminary Discussion on Establishing Animal Model of Warm-dryness Syndrome of Lung Injury by Haze

目的模拟温燥环境,制作雾霾伤肺温燥证大鼠模型,初步探讨雾霾伤肺温燥证动物模型的制作方法。方法将20只Wistar大鼠随机分为空白对照组、温燥模型组,每组10只。在人工设置温燥环境下,采用自制PM2.5(Particular Matter,空气动力学直径≤2.5 m的颗粒物)烟雾染毒箱对温燥模型组大鼠进行染毒,通过对大鼠行为学观察、血清炎性因子检测、肺组织病理切片分析对模型进行评价。结果造模4周后温燥模型组大鼠出现声音嘶哑、躁动、毛发枯槁、口腔分泌物减少、喜饮等表现;肺组织切片可见炎性损伤,出现肺泡间隔增宽、肺组织结构破坏、成纤维细胞增殖、大量胞外基质聚集,而空白组无明显异常;血清检测提示温燥模型组炎性因子白介素-1(IL-1)表达水平明显增高,与空白对照组比较具有显著性差异(P<0.01);白介素-8(IL-8)表达水平增高,与空白对照组比较差异不明显(P>0.05)。结论本实验模拟温燥环境,建立雾霾伤肺温燥证动物模型的实验方法可行。

Objective To make a rat model of a warm-dryness syndrome of lung injury by haze in a simulated warm-dry environment,and to explore the method of making the model of warm-dryness syndrome of lung injury by haze.Methods 20 Wistar rats were randomly divided into blank control group and warm-dry model group,each group of 10.In the artificially set warm-dry environment,the rats in warm-dry model group were poisoned by using a self-made PM2.5 smoke poisoning box.The model was evaluated by means of observations of behavior,detection of inflammatory factors in serum,and pathological analysis of lung tissue.Results 4 weeks after modeling,the rats in model group showed hoarseness,restlessness,haggard hair,decreased oral secretions and liked drinking.The lung tissue showed inflammatory damage,the alveolar septum of rats in warm dry model group was widened,structural damage,myofibroblasts proliferated,large amount of extracellular matrix concentration.The expression of interleukin-1(IL-1)in the serum of warm-dry model group was significantly increased.Compared with blank control group,there was a significant difference(P<0.01);the expression level of IL-8 increased and the difference between the two groups was not significant(P>0.05).Conclusion The experimental method of establishing animal model of warm-dryness syndrome of lung injury by haze is feasible.