烟草花粉活力检测方法筛选

Screening on the methods for detecting the pollen viability of Nicotiana

为了探究出一种快速、准确测定烟草花粉活力的方法,研究以烟草栽培品种Nicotiana tabacum L’K326’、残波烟草(N.repanda)和黄花烟草(N.rustica)的成熟花粉为材料,运用离体萌发法、FDA-PI荧光染色法、TTC染色法、I2-KI染色法以及联苯胺染色法分别对上述烟草的花粉活力进行检测.结果表明:离体萌发法是检测烟草花粉活力最准确有效的方法;TTC染色法可以用于烟草花粉活力的快速检测;FDA-PI荧光染色法、I2-KI染色法和联苯胺染色法则不适用于烟草花粉活力的检测.

To determine a rapid and accurate method in pollen viability detection of Nicotiana,in vitro germination was applied in testing the viability of the mature pollen stained with FDA-PI,TTC,I2-KI and Benzidine from Nicotiana tabacum L’K326’,N.repanda and N.rustica.The results demonstrated that the most accurate and effective method was in vitro germination combining TTC method,which could detect the pollen viability rapidly,while the other three staining methods were not applicable to the pollen viability detection of Nicotiana.