新型冠状病毒肺炎确诊病例粪便标本的病毒核酸检测

Nucleic acid detection of fecal samples from confirmed cases of COVID-19

目的了解新型冠状病毒肺炎(COVID-19)确诊病例粪便中2019-nCoV感染情况。方法收集36例确诊病例的粪便标本/肛拭子标本,采用实时荧光RT-PCR检测2019-nCoV载量,利用统计分析软件SPSS 19.0比较病例的带毒率。结果36份标本共检出2019-nCoV核酸阳性20份(55.56%),危重症病例(2/3)和重症病例标本阳性率均为66.67%(6/9)、普通肺炎病例阳性率为62.50%(10/16)、轻症肺炎病例阳性率为25.00%(2/8)。36例确诊病例包括男性22例、女性14例,检出率分别为54.55%和57.14%;病例年龄分布于17~86岁之间,平均为48.75岁。36份标本其中5份肛拭子标本检出阳性标本2份,31份粪便标本检出阳性标本18。临床分型、性别、年龄和标本类型各组间阳性检出率均无统计学差异。结论本研究发现COVID-19患者,无论重症、轻症患者粪便标本均存在2019-nCoV基因检测阳性,提示消化道可能是该病毒排泄渠道,因此可能存在粪-口传播途径,研究结果具有重要的临床和流行病学意义。

To investigate the status of viral infection in the feces of confirmed COVID-19,cases fecal samples or anal swabs from 36 confirmed cases were collected,2019-nCoV was detected by real-time fluorescence RT-PCR,and the infection rates of the cases were compared by statistical analysis software SPSS 19.0.Among the 36 samples,20(55.56%)were positive for 2019-nCoV.The positive rate of critical cases(2/3)and severe cases(6/9)were both 66.67%,the positive rate of common pneumonia was 62.50%(10/16),and the positive rate of mild pneumonia was 25.00%(2/8).The 36 confirmed COVID-19 cases included 22 males and 14 females,with a detection rate of 54.55%and 57.14%,respectively.The age distribution range of the cases was ranging between 17 and 86 years old,with an average age of 48.75 years.Among the 36 samples,2 positive specimens in 5 anal swab specimens and 18 positive specimens in 31 fecal specimens were detected.There were no statistically significant differences in the positive detection rates among clinical types,genders,ages and specimen types,respectively.From these results,we deduced that fecal samples of confirmed COVID-19 cases contained 2019-nCoV,which can cause infection through potential fecal-oral transmission and be significant for clinical treatment and epidemiological study.