蒲公英酶解抗菌肽的分析

Analysis of Antimicrobial Peptides in Taraxacum mongolicum Enzymatic Hydrolysis

为拓展蒲公英抗菌肽在抗菌类新药领域的开发应用,利用酶解法制备蒲公英抗菌肽对比蒲公英蛋白大于10 kD组分、3~10 kD组分、1~3 kD组分、小于1 kD组分的抑菌活性,根据筛选结果对抑菌活性较强的组分使用LC-MS技术进行结构解析来分析药效物质基础。结果表明:各组分对大肠杆菌和金黄色葡萄球菌的生长抑制活性顺序为酶解肽>小于1 kD组分>1~3 kD组分>3~10 kD组分>大于10 kD组分>空白对照。推断分析小于1 kD组分结构发现3个寡肽:PGYGT-T1的结构为NH2-Asp-Val-COOH,PGYGT-T2的结构为NH2-Asn-Glu-COOH,PGYGT-T3的结构为NH2-Val-Arg-COOH;推断分析酶解肽结构发现3个寡肽:PGYGT-M1的结构为NH2-PHe-Lys-COOH或NH2-PHe-Gln-COOH,PGYGT-M2的结构为NH2-His-Cys-COOH,PGYGT-M3的结构为NH2-Val-Arg-COOH,结果说明蒲公英抑菌活性最好的酶解肽组分和小于1 kD组分药效物质均为寡肽类成分。该研究为蒲公英蛋白肽的开发利用及蒲公英抗菌肽新药的生产提供帮助。

To extend the Taraxacum mongolicum antibacterial peptide antibiotic class in the field of new drug development and application,comparison of antibacterial peptide enzyme hydrolysis Taraxacum mongolicum Taraxacum mongolicum is more than 10 kD protein components,3~10 kD components,1~3 kD components,less than 1 kD bacteriostatic activity of components.According to the results of screening for strong bacteriostatic activity of components using LC-MS technique for structural analysis to analyze the efficacy material base.The results showed that the growth inhibitory activities of each component on Escherichia coli and Staphylococcus aureus were in the order of enzymatic hydrolysis peptide>less than 1 kD component>1~3 kD component>3~10 kD component>greater than 10 kD component>blank control.The results showed that the structure of PGYGT-T1 was NH2-Asp-Val-COOH,the structure of PGYGT-T2 was NH2-Asn-Glu-COOH,and the structure of PGYGT-T3 was NH2-Val-Arg-COOH.The structure of PGYGT-M1 was NH2-Phe-Lys-COOH or NH2-PHe-Gln-COOH,the structure of PGYGT-M2 was NH2-His-Cys-COOH,and the structure of PGYGT-M3 was NH2-Val-Arg-COOH.This research is helpful for the development and utilization of Taraxacum mongolicum protein peptides and the pro-duction of new Taraxacum mongolicum antimicrobial peptides.