FOXJ1及SATB-1蛋白在三阴性乳腺癌组织中的表达及其对预后的影响

Expression of FOXJ1 and SATB-1 protein in triple-negative breast cancer and their influence on prognosis

目的探讨叉头框蛋白J1(FOXJ1)与核基质结合区蛋白质1(SATB-1)在三阴性乳腺癌组织中的表达以及对预后的意义。方法收集2007年7月至2014年7月进行外科手术的100例三阴性乳腺癌患者的癌组织标本以及对应的100例癌旁组织标本。并采用免疫组化染色法检测癌组织与癌旁组织中FOXJ1、SATB-1蛋白表达水平,分析二者与三阴性乳腺癌临床病理特征的关系,采用Cox回归分析探讨三阴性乳腺癌患者预后影响因素。结果癌组织FOXJ1、SATB-1蛋白阳性表达率分别为40.00%、58.00%;癌旁组织FOXJ1、SATB-1蛋白阳性表达率分别为62.00%、39.00%,两组FOXJ1、SATB-1蛋白阳性表达比较有统计学差异(P<0.01)。组织学Ⅱ~Ⅲ级、肿瘤低分化、淋巴结转移的患者FOXJ1阴性表达率、SATB-1阳性表达率均较高(P<0.01)。FOXJ1阳性和阴性表达的中位生存期分别为52个月、30个月;SATB-1阳性和阴性表达中位生存期分别为31个月、53个月。单因素分析显示,组织学Ⅱ~Ⅲ级、肿瘤低分化、有淋巴结转移、FOXJ1阴性表达及SATB-1阳性表达的三阴性乳腺癌患者生存时间均显著缩短(P<0.01)。多因素Cox回归分析显示,组织学Ⅱ~Ⅲ级、肿瘤低分化、淋巴结转移、FOXJ1阴性表达、SATB-1阳性表达均为影响三阴性乳腺癌预后的独立危险因素(P<0.01)。结论FOXJ1在三阴性乳腺癌组织中表达水平较低,而SATB-1表达水平较高,二者与肿瘤的组织学分级、肿瘤分化程度、淋巴转移等病理变化密切相关,可作为预测三阴性乳腺癌预后的重要指标。

Objective To investigate the expression of forkhead-box J1(FOXJ1) and special AT-rich sequence-binding protein-1(SATB-1) in triple-negative breast cancer(TNBC) tissue and their influence on prognosis.Methods The cancer tissue samples of 100 TNBC patients who received surgery from July 2007 to July 2014,and their corresponding adjacent tissue samples were collected.The expression levels of FOXJ1 and SATB-1 in cancer and adjacent tissues were detected by immunohistochemistry,and the relationship between them and the clinicopathological characteristics of TNBC was analyzed.The prognostic factors of TNBC patients were analyzed by Cox regression analysis.Results The positive rates of FOXJ1 and SATB-1 were 40.00% and 58.00% in cancer tissues,62.00% and 39.00% in adjacent tissues,respectively.There was significant difference in the positive expression of FOXJ1 and SATB-1 between cancer and adjacent tissues(P<0.01).The negative expression rate of FOXJ1 and the positive expression rate of SATB-1 in patients with grade Ⅱ-Ⅲ,low differentiation and lymph node metastasis were significantly higher(P<0.01).The median survival time of FOXJ1 positive and negative expression was 52 months and 30 months respectively,and that of SATB-1 positive and negative expression was 31 months and 53 months respectively.Single-factor analysis showed that the survival time of TNBC patients with grade Ⅱ-Ⅲ,low differentiation of tumor,lymph node metastasis,FOXJ1 negative expression and SATB-1 positive expression was significantly shorter(P<0.01).Multivariate Cox regression analysis showed that grade Ⅱ-Ⅲ,low differentiation of tumor,lymph node metastasis,FOXJ1 negative expression and SATB-1 positive expression were independent risk factors for prognosis of TNBC(P<0.01).Conclusion FOXJ1 expression is low and STAB-1 expression is high in TNBC tissues and they are closely related to pathological changes such as tumor histological grade,tumor differentiation degree,lymph node metastasis,and can be used as important indicators to predict the prognosis of TNBC.