血清骨桥蛋白和肝癌的关系可能归因于乙型肝炎病毒慢性感染

The association of osteopontin and liver cancer may be attributable to chronic hepatitis B virus infection

目的研究乙型肝炎病毒(HBV)感染是否增加血清骨桥蛋白(OPN)浓度及OPN与肝癌之间的关系。方法按病例对照研究原理,研究对象分为6个组:HBsAg无症状携带者组(ASC组)、HBsAg阳性慢性肝病组、HBsAg阳性肝癌组、HBsAg阴性其他肿瘤组、HBsAg阴性肝癌和慢性肝脏病组、健康对照组。用化学发光微粒子免疫检测法检测血清HBsAg浓度,用酶联免疫吸附试验(ELISA)检测血清OPN浓度,用Mann-Whitney U检验和线性回归分析法进行统计分析。结果6组研究对象共241名。HBsAg阳性肝癌组与HBsAg阴性其他肿瘤组、健康对照组血清OPN浓度差异均有统计学意义(P值均=0.0001)。在HBsAg阳性肝癌组与ASC组、HBsAg阳性慢性肝病组间,以及在HBsAg阴性肝癌和慢性肝脏病组与健康对照组间,血清OPN浓度差异均无统计学意义(P值分别为0.360、0.588和0.075)。多元线性回归分析结果显示,血清HBsAg浓度是与OPN浓度有关的唯一变量(P=0.0001)。结论血清OPN浓度可能与肝癌本身无关,而与HBV慢性感染有关,在HBV感染者中,可以用血清HBsAg浓度预测血清OPN浓度。

Objective To determine whether infection with hepatitis B virus(HBV)increases the serum concentration of osteopontin(OPN)and its association with hepatocelluar carcinoma(HCC).Methods A casecontrol study was designed.The study subjects comprised of six groups:HBsAg asymptomatic groups(ASC group),HBsAg(+)with chronic liver disease group,HBsAg(+)with HCC group,HBsAg(-)with other tumours group,HBsAg(-)with HCC or chronic liver disease group,and the healthy control group.The concentration of serum HBsAg was measured by commercial chemiluminescent microparticle immunoassay.The concentration of serum OPN was measured by enzyme-linked immunosorbent assay(ELISA).The data was statistically analyzed by Mann-Whitney U test and linear regression.Results Two hundred and forty-one subjects were divided into six groups.The difference between the concentrations of OPN in the HBsAg(+)with HCC group,HBsAg(-)with other cancers group,and the healthy control group were statistically significant(all P values were 0.0001).The difference between the concentrations of OPN in HBsAg(+)with HCC group and ASC group,HBsAg(+)with HCC group and HBsAg(+)with chronic liver disease group,and HBsAg(-)with HCC or chronic liver disease group and the healthy control weren’t statistically significant(P=0.360,0.588,and 0.075,respectively).Multiple linear regression analysis showed that the serum concentration of HBsAg was the only variable independently associated with the serum concentration of OPN(P=0.0001).Conclusions The concentration of osteopontin serum may not be attributable to liver cancer,but attributable to chronic HBV infection.The serum concentration of HBsAg may be used to predict the serum concentration of OPN among patients with HBV infection.