氨基酸分子印迹传感器的制备及其在手性识别中的应用

Preparation of amino acid and molecularly imprinted sensor and its application in chiral recognition

【目的】通过构建分子印迹传感器对不同构型的色氨酸分子进行手性识别,探讨该传感器的手性识别能力及实际应用效果.【方法】以石墨烯为基底,利用分子印迹法制备色氨酸分子印迹传感器,探究不同pH值、沉积时间和色氨酸浓度对该传感器识别色氨酸对映体能力的影响,并通过配制不同浓度的L-色氨酸和D-色氨酸溶液,检测氧化峰电流值的差值ΔI,探究浓度与ΔI的关系.【结果】5.0 mmol/L色氨酸对映体在分子印迹传感器中的I_D/I_L为4.36,说明传感器对L-色氨酸有很好的识别能力.当电解液的pH值等于6.0,沉积时间为5 min时,所制备手性传感器性能最佳,并且ΔI与色氨酸浓度满足良好的线性关系,表明在实际应用时该传感器具有良好的准确性和灵敏度.【结论】色氨酸分子印迹传感器制作简单,检测灵敏,结果准确,为手性识别氨基酸提供了高效的方法.

【Objective】 By constructing a molecularly imprinted sensor for the chiral recognition of tryptophan molecules with different configurations,the chiral recognition ability of the sensor and its practical application effect were studied.【Method】 Graphene was used as a substrate material and tryptophan molecularly imprinted sensor was prepared by molecularly imprinting.The effects of pH value,deposition time and L-tryptophan concentration on the electrode properties were explored.Meanwhile,the effects of different concentrations of L-and D-tryptophan solution concentration to peak current were discussed.【Result】 The I_D/I_L of 5.0 mmol/L tryptophan enantiomer in molecularly imprinted sensor was 4.36,indicating that the sensor performed good recognition ability for L-tryptophan.The performance of the graphene/poly-lysine chiral recognition electrode was the best when the pH of electrolyte was 6 and deposition time was 5 min.In addition,the linear relationship between ΔI and tryptophan concentration shown that the accuracy and sensitivity of sensor were satisfied in practical application.【Conclusion】 The tryptophan molecularly imprinted sensor is an efficient method for the chiral recognition of amino acids with high sensitive and accurate,and easy preparation procedure.