针刺对应激性胃溃疡模型大鼠肠道菌群的影响

Effect of acupuncture on intestinal flora in rats with stress gastric ulcer

目的:观察针刺"百会""中脘"和"足三里"对应激性胃溃疡(SGU)模型大鼠肠道菌群的影响,探讨针刺促进SGU恢复的机制。方法:将31只SPF级SD大鼠随机分为空白组(7只)、模型组(8只)、针刺组(8只)和药物组(8只)。模型组、针刺组及药物组大鼠选用束缚-浸水法的改良办法制备SGU模型。造模后,空白组和模型组每天固定束缚20 min,共5 d;针刺组选取大鼠"百会""中脘""足三里"穴针刺干预,每日1次,每次20 min,每5分钟捻转行针30 s,共5 d;药物组选用奥美拉唑肠溶片溶液(200 mg/mL)灌胃,每只2 mL,每日1次,共5d。应用Guth法计算胃黏膜损伤指数,HE染色法观察胃黏膜形态学改变,16Sr DNA测序法检测肠道菌群结构丰度。结果:与空白组比较,模型组大鼠胃黏膜损伤指数升高(P<0.01),大鼠胃黏膜病理改变明显,肠道菌群丰富度指数Chao1、Observed species及多样性指数Shannon均降低(P<0.05),多样性指数Simpson升高(P<0.05);与模型组比较,针刺组、药物组大鼠胃黏膜损伤指数均降低(P<0.01,P<0.05),胃黏膜病变程度减轻,肠道菌群丰富度指数Chao1、Observed species及多样性指数Shannon均升高(P<0.05),多样性指数Simpson降低(P<0.05);与药物组比较,针刺组大鼠胃黏膜损伤指数降低(P<0.01),胃黏膜恢复程度优于药物组。结论:针刺能够有效改善SGU引发的胃黏膜损伤,其机制可能与增加菌群多样性,促使紊乱的肠道菌群水平回调有关。

Objective To observe the effect of acupuncture at "Baihui"(GV 20), "Zhongwan"(CV 12) and "Zusanli"(ST 36) on intestinal flora in rats with stress gastric ulcer(SGU), and to explore the mechanism of acupuncture promoting SGU recovery. Methods Thirty-one SPF SD rats were randomly divided into a control group(7 rats), a model control group(8 rats), an acupuncture group(8 rats) and a medication group(8 rats). The rats in the model group, acupuncture group and medication group were selected to applied the improved restraint water-immersion stress method to establish the SGU model. After modeling, the rats in the control group and model group were fixed and restrained for 20 min every day for a total of 5 days;the rats in the acupuncture group were intervented with acupuncture at "Baihui"(GV 20), "Zhongwan"(CV 12) and "Zusanli"(ST 36), once a day, 20 min each time, and twisting needle for 30 s every 5 min for a total of 5 days;the rats in the medication group were gavaged by solution of omeprazole enteric-coated tablet(200 mg/mL), 2 mL for each rat, once a day. Guth method was used to calculate the gastric mucosal damage index(GMDI), HE staining was used to observe the pathological changes of gastric mucosa, and 16 SrDNA identification was used to detect the structural abundance of intestinal flora. Results Compared with the control group, the GMDI of rats in the model group was increased(P<0.01), the gastric mucosal pathological changes were significant, and the intestinal flora richness index Chao1, Observed species and diversity index Shannon were all decreased(P<0.05), the diversity index Simpson was increased(P<0.05). Compared with the model group, the GMDI of rats in the acupuncture group and medication group was reduced(P<0.01, P<0.05), the gastric mucosal damage degree was reduced, and the intestinal flora richness index Chao1, Observed species and diversity index Shannon were all increased(P<0.05) and the diversity index Simpson decreased(P<0.05). Compared with the medication group, the GMDI of rats in the acupuncture group was reduced(P<0.01), the recovery of gastric mucosal injury was better than that of the medication group. Conclusion Acupuncture can effectively improve gastric mucosal injury of SGU, and the mechanism may be related to increasing the diversity of intestinal flora and promoting the correction of the disordered intestinal flora.