不同浓度七氟烷对大鼠高氧性肺损伤的影响

Effects of sevoflurane on hyperoxia-induced lung injury in rat

目的研究不同浓度七氟烷对大鼠高氧性肺损伤的影响。方法SD大鼠72只,随机分为对照组C组(n=12)、七氟烷吸入组S组,S组包含5个亚组(n=12)S0、S1.0、S1.5、S2.0、S2.5。C组不作任何处理,S组大鼠采用95%浓度氧气吸入48 h法制作高氧性肺损伤模型,造模后各亚组大鼠分别吸入浓度为0%、1.0%、1.5%、2.0%、2.5%的七氟烷1 h;C组自由呼吸空气1 h。在完成高氧处理48 h时(T1)、七氟烷吸入1 h时(T2)两个时间点,每组各取6只大鼠,经腹主动脉采集动脉血做血气分析;用酶联免疫吸附试验检测血清肿瘤坏死因子(TNF)-α、白细胞介素(IL)-8和IL-6浓度;取右肺组织行HE染色后光镜观察病理学改变并进行肺损伤评分,取左肺组织测定湿/干比值(W/D)。结果在高氧处理48 h后(T1):与C组比较,S组PaO2降低,PaCO2、TNF-α、IL-8和IL-6浓度、W/D比值升高,病理损伤评分升高(P<0.05),S0至S2.5组组间两两比较,上述指标均无统计学差异;经七氟烷处理1 h后(T2):与C组比较,S组PaO2降低,PaCO2、TNF-α、IL-8和IL-6浓度、W/D比值、病理损伤评分升高(P<0.05);与七氟烷处理前比较,S1.0至S2.5组PaO2升高,PaCO2、TNF-α、IL-8和IL-6浓度、W/D比值、病理损伤评分降低(P<0.05),S0组上述指标无统计学差异;经七氟烷处理后,与S2.0组比较,S1.0和S1.5组的PaO2降低,PaCO2、TNF-α、IL-8和IL-6浓度、W/D比值、病理损伤评分升高(P<0.05),S2.5组上述指标无统计学差异。结论七氟烷可以有效降低大鼠高氧导致的肺损伤程度,以2%浓度七氟烷肺保护效果最佳。

Objective To explore the effects of different concentrations of sevoflurane on hyperoxia-induced lung injury in rat.Methods The 72 SD rats were randomly divided into control group C(n=12);sevoflurane inhalation group S,group S contains 5 subgroups(n=12)S0,S1.0,S1.5,S2.0,S2.5.Group C wasn’t given any treatment,rats in group S were inhaled 95%oxygen for 48 hours to establish a hyperoxia-induced lung injury model.then rats in each subgroup inhaled sevoflurane at different concentrations of 0%,1.0%,1.5%,2.0%and 2.5%for 1 h respectively,rats in group C were breathe air freely.At the two time points which include inhaled 95%oxygen for 48 hours,and sevoflurane was inhaled for 1 h,blood was collected by the abdominal aorta,then arterial blood was used for blood gas analysis;using enzyme linked immunosorbent assay for the detection of serum tumor necrosis factor(TNF)-αand interleukin(IL)-8 and IL-6 concentration;HE staining was carried out in the right lung,and the pathological changes of lung tissue were observed under light microscope;Wet to dry ratio(W/D)of the left lung was taken.Results After inhalation of 95%oxygen for 48 hours(T1):compared with the group C,group S of arterial blood gas results suggested that the PaO2 value decreased,PaCO2 value increased.The degree of lung tissue injury and the pathological score,TNF-α,IL-8 and IL-6,W/D content increased significantly(P<0.05),there was no significant difference between the S0 to S2.5 groups;After treatment with sevoflurane for 1 h(T2):compared with the group C,group S of arterial blood gas results suggested that the PaO2 value decreased,PaCO2 value increased.the degree of lung tissue injury and the pathological score,TNF-α,IL-8 and IL-6,W/D content increased significantly(P<0.05);Compared with before sevoflurane treatment,the PaO2 value increased,PaCO2 value decreased,TNF-α,IL-8 and IL-6,W/D content decreased,pathological score decreased in group S1.0 to S2.5(P<0.05),but there was no significant difference in group S0;After treatment with sevoflurane,compared with S2.0 group,the PaO2 value decreased,PaCO2 value increased,TNF-,IL-8 and IL-6,W/D content increased,pathological score increased in the group S1.0 and S1.5(P<0.05),but there was no significant difference in group S2.5.Conclusion Sevoflurane can effectively reduce the degree of lung injury caused by hyperoxia in rats especially when the concentration is 2%.