摘要
采用糙米培养基对川芎内生菌Fusarium tricinctum菌种进行扩大培养,利用萃取、柱色谱、制备色谱等手段对其化学成分进行分离纯化,并通过NMR、ESI-MS等波谱手段对所得化合物进行结构鉴定;采用MTT法对部分化合物进行细胞毒活性测定.共分离得到13个化合物,分别鉴定为苯乙酸(1),3,6-二异丙基-1-甲基哌嗪-2,5-二酮(2),fusarpyroneA(3),fusagerinB(4),N-苯乙基乙酰胺(5),fungerin(6),callyspongidipeptideA(7),环-(S-脯氨酸-R-亮氨酸)(8),vomifolilol(9),环-(4-S-羟基-R-脯氨酸-R-异亮氨酸)(10),N-acetyl-β-oxotryptamine(11),烟酸(12),邻羟基苯乙酸(13).化合物6对OCI-LY10、HT29、MV4-11、Hela、SKOV3具有一定的抑制作用,IC50值分别为23.98±2.37、93.63±1.42、90.64±1.65、77.65±1.35、52.52±2.04μmol/L.上述结果可为该菌种的肿瘤细胞毒活性开发提供一定的研究依据.(表1参28)
To investigate the compounds produced by the endophytic fungus Fusarium tricinctum from Ligusticum chuanxiong Hort.and their cytotoxicity against three tumor cell lines,F.tricinctum was cultured with brown rice and its secondary metabolites were purified by chromatographic procedures and identified based on NMR,ESI-MS,and other spectral evidence.The MTT method was applied to test the cytotoxicity of the isolated compounds.A total of 13 compounds were isolated and identified as phenylacetic acid(1),3,6-diisopropyl-1-methylpiperazine-2,5-dione(2),fusarpyrone A(3),fusagerin B(4),N-phenethylacetamide(5),fungerin(6),callyspongidipeptide A(7),cyclo-((S)-Pro-(R)-Leu)(8),vomifolilol(9),cyclo-(4-S-hydroxy-R-proline-Risoleucine)(10),N-acetyl-β-oxotryptamine(11),nicotinic acid(12),and 2-hydroxybenzeneacetic acid(13).Fungerin showed weak inhibitory activity against OCI-LY10,HT29,MV4-11,Hela,and SKOV3 cells,with IC50 values of 23.98±2.37,93.63±1.42,90.64±1.65,77.65±1.35,and 52.52±2.04μmol/L,respectively.
作者
曹钰镁
金梦莹
谭璐
罗禹
胡靖文
赵佳佳
郭大乐
邓赟
CAO Yumei;JIN Mengying;TAN Lu;LUO Yu;HU Jingwen;ZHAO Jiajia;GUO Dale;DENG Yun(Key Laboratory of Standardization of Chinese Herbal Medicine,Ministry of Education,Sichuan Provincial Key Laboratory of Systematic Research,Development and Utilization of Chinese Medical Resources:Ministry of State Key Laboratory Breeding Base,School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;School of Pharmacy,Southwest Medical University,Luzhou 646000,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2020年第2期294-298,共5页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金面上项目(81373961)
四川省科技厅省青年科技创新研究团队专项计划(2016TD0006)
中国博士后科学基金(2017M622985)
四川省教育厅科研项目(18ZA0191)
成都中医药大学青年教师创新基金(ZRQN1766)资助。