基于光学相干层析的生物3D打印人工皮肤量化分析

Quantitative Analysis of Biological 3D Printed Artificial Skin Using Optical Coherence Tomography

对人工皮肤3D特征的无损定量检测分析,是研究皮肤打印和诱导培养技术须解决的关键问题。利用谱域光学相干层析成像(SD-OCT),对生物3D打印人工皮肤进行无损成像和量化分析。基于光学相干层析成像(OCT)强度信号的自适应峰值检测算法,量化皮肤3D厚度分布和粗糙度变化,通过计算不同位置的皮肤整体厚度及其波动,定量可视化皮肤的空间分辨结构特征。OCT成像人工皮肤的结构特征与切片的苏木精伊红(H&E)染色结果一致,两者实测皮肤整体厚度差异最大为3.59μm,可验证该方法的可行性和准确性。通过SD-OCT对人工皮肤在培养周期内的持续检测,2D人工皮肤厚度分布检测可以展示皮肤在不同培养时间的厚度增长曲线,而3D空间分辨厚度分布图和表面粗糙图谱能更直观地显示皮肤生长状态。量化统计结果表明,在气液培养过程中,人工皮肤的整体平均厚度不断增长并趋于稳定,皮肤成熟时整体平均厚度为83.91μm;皮肤表面粗糙度随角质化程度变化先减后增。基于OCT强度信号量化分析的方法能真实有效反映生物3D打印人工皮肤的结构参数变化,可为人工皮肤制备过程中的质量评估提供一种可靠的监测手段。

The non-destructive quantitative detection and analysis of the artificial skin three-dimensional features is a key problem to be solved in the research of skin printing and induced culture technology.This paper used spectral domain optical coherence tomography(SD-OCT)to perform non-destructive imaging and quantitative analysis on biological 3D printed artificial skin.The adaptive peak detection algorithm based on OCT intensity signal quantified skin three-dimensional thickness distribution and roughness variation.The overall thickness of the skin at the location and its fluctuation quantitatively visualized the spatially resolved structural features of the skin.The structural characteristics of OCT imaging artificial skin were consistent with the results of sliced hematoxylin and eosin(H&E)staining.The difference between the two measured skin thicknesses was only 3.59μm,which verified the feasibility and accuracy of the method.Through the continuous detection of artificial skin in the culture period by SD-OCT,the two-dimensional artificial skin thickness distribution detection can show the thickness growth curve of the skin at different culture time,and the three-dimensional spatial resolution thickness distribution map and surface roughness map can be more visually show skin growth status.Quantitative statistical results show that during the gas-liquid culture,the overall average thickness of the artificial skin is constantly increasing and stabilizes.When the skin matures,the overall average thickness is 83.91μm.The surface roughness of the skin first decreases and then increases with the change of keratinization.Therefore,the method based on OCT intensity signal quantitative analysis can truly and effectively reflect the structural parameter changes of biological 3D printed artificial skin,which provides a reliable monitoring method for quality assessment in artificial skin preparation.