淡豆豉异黄酮的提取分离及对DPPH自由基清除能力的研究

The Isoflavone Extraction and Separation of SSP and Their Capacity of DPPH Radical Scavenging

目的:考察纯种发酵淡豆豉中异黄酮的最佳提取和纯化工艺,并考察异黄酮提取物的体外抗氧化能力。方法:以异黄酮中的主要有效成分大豆黄素和染料木素含量为指标,采用正交试验优化淡豆豉异黄酮的超声辅助提取工艺和大孔树脂纯化工艺,并考察异黄酮体外清除DPPH自由基的能力。结果:淡豆豉异黄酮超声辅助提取最佳工艺为超声时间90 min,酶量10 U,料液比1∶10,在此条件下淡豆豉中异黄酮的提取率为0.598%。使用HPD-100型大孔树脂进行分离纯化效果最好。在DPPH自由基清除实验中,淡豆豉异黄酮提取物IC50(半数有效抑制浓度)为8.18μg/mL,BHA为15.79μg/mL。结论:按本实验结果提取的淡豆豉异黄酮提取率较高,且提取物具有显著的DPPH自由基清除效果。

Objective:To investigate the best extracted and purified method for isoflavones in SSP by using pure breed fermentation and considering the antioxidant capacity in vitro.Methods:The ultrasonic assisted extraction process and macroporous resin purification process of isoflavones in fermented soya bean were optimized by orthogonal test,and the ability of isoflavones to remove DPPH free radicals in vitro was investigated.Results:The optimal ultrasound-assisted extraction process of isoflavones from fermented black beans was 90 min,10 U enzyme content and 1∶10 solid-liquid ratio.Under these conditions,the extraction rate of isoflavones from fermented black beans was 0.598%.HPD-100 macroporous resin showed the best separation and purification effect.In the DPPH free radical scavenging experiment,the IC50(half inhibition rate)value of isoflavone extract of soya beans was 8.18μg/mL,while the positive control BHA was 15.79μg/mL.The isoflavone extract of soya beans had significant DPPH free radical scavenging effect.