限制性两阶段多位点全基因组关联分析法(RTM-GWAS)的特点、常见提问与应用前景

Major Characteristics,Often-Raised Queries and Potential Usefulness of the Restricted Two-Stage Multi-Locus Genome-Wide Association Analysis

限制性两阶段多位点全基因组关联分析方法(RTM-GWAS)是新建立的一种可以全面检测自然群体和双(多)亲衍生群体中具有不同复等位变异QTL体系的关联分析方法。本文介绍了提出RTM-GWAS的出发点及其两大主要特点,包括建立适合自然群体和和双(多)亲衍生群体特点的复等位变异标记和控制总体贡献率的多位点关联分析模型。一般读者和编者对RTM-GWAS的方法、原理,对复等位标记和多位点模型并无异议,提问与质疑主要分为两方面:一是RTM-GWAS检测到的QTL数量较多,大大多于单位点MLM模型所检出的QTL数目,怀疑增加的QTL是假阳性所致;另一是采用常规显著水准要求太低,不适于关联分析。本文对此做了严密释疑。最后介绍了关于RTM-GWAS的应用前景,包括遗传体系解析与重要基因克隆,双(多)亲杂交衍生群体遗传解析,群体遗传分化与进化和设计育种等方面。

Restricted two-stage multi-locus genome-wide association analysis(RTM-GWAS)is a novel GWAS procedure which provides a way to identify the QTL system with various multiple alleles in natural and bi-or multi-parental derived populations.The major purposes and its two major characteristics of the RTM-GWAS procedure were presented,including the establishment of the SNPLDB markers with multiple alleles fitting the property of the natural and bi-or multi-parental derived populations and the establishment of multi-locus model GWAS procedure with the total genetic contribution controlled within heritability value.Generally,the readers and editors do not doubt about the methods and principles,the multiple allele markers and the multi-locus model,but have questions and queries on the large amount of detected QTLs many more than those from single locus MLM-GWAS procedure and on the general significance level without correction used in RTM-GWAS.These doubts were carefully and seriously explained and relieved.Furthermore,the potential usefulness of the RTM-GWAS procedure in genetic and evolutionary studies were summarized,including usefulness in relatively thorough identification of the QTL-allele system in populations and major gene finding and cloning,usefulness in relatively thorough identification of the QTL-allele system in bi-and multi-parental derived populations,usefulness in studies on population genetic differentiation and evolution and usefulness in breeding by genetic design.