DK-β、Lgr5和mTOR/P70s6k在胃癌中的表达及对预后的预测价值

Expressions of DK-β,Lgr5 and mTOR/P70s6k in gastric cancer and their predictive value in evaluating prognosis of patients

目的研究DK-β、Lgr5和mTOR/P70s6k在胃癌中的表达及对预后的预测价值。方法选择2016年6月份至2018年6月份收治的85例胃癌患者为研究对象,并选取同期80例非胃癌患者为对照组,取患者胃癌组织及癌旁组织以及对照组胃黏膜组织进行RT-PCR及免疫组织化学SP法检测DK-β、Lgr5、mTOR、P70s6k在胃癌中的表达。比较其表达指标,阳性率以及生存率关系。结果DK-β、Lgr5、mTOR、P70s6k在胃癌组织的表达指标明显高于癌旁组织及对照组,差异有统计学意义(P<0.05)。DK-β、Lgr5、mTOR、P70s6k的表达比较呈正相关性(P<0.05)。Lgr5、mTOR、P70s6k在3组组织中的阳性表达胃癌组织阳性率,明显高于癌旁组织以及对照组;DK-β阳性率的表达中癌旁组织的阳性率较胃癌组织以及对照组比显著增高,差异有统计学意义(P<0.05)。DK-β、Lgr5、mTOR、P70s6k在胃癌中的表达表达与胃癌患者年龄、性别比较差异无统计学意义(P>0.05)。DK-β在胃癌患者肿瘤直径大小阳性率的表达差异无统计学意义(P>0.05)。Lgr5、mTOR、P70s6k阳性率的表达在胃癌患者肿瘤直径<5 cm(60.41%,58.33%,56.52%)与直径≥5 cm的阳性率(86.48%,75.68%,72.97%)比较差异有统计学意义(P<0.05)。DK-β、Lgr5、mTOR、P70s6k在TNM分期,组织学分级,淋巴转移情况比较差异有统计学意义(P<0.05)。结论DK-β、Lgr5和mTOR/P70s6k在胃癌中的表达中对患者的肿瘤TNM分期,组织学分级,淋巴转移情况有密切的关系,其中Lgr5和mTOR/P70s6k的高、低表达对患者的预后生存情况有一定的预测评估价值,DK-β则可以作为早期的筛查手段,对患者早期血清中的检测可以进行筛选。

Objective To investigate the expressions of DK-β,Lgr5 and mTOR/P70s6k in gastric cancer and their predictive value in evaluating prognosis of patients with gastric cancer.Methods A total of 85 patients with gastric cancer who were admitted and treated in our hospital from May 2014 to August 2015 were enrolled as subjects,at the same time,the other 80 patients with non-gastric cancer were enrolled as control group.The protein expression levels of DK-β,Lgr5,mTOR and P70s6k were detected by RT-PCR and immunohistochemical SP assay in gastric cancer tissues and paracancer tissues as well as gastric mucosal tissues in control group.Moreover the other indexes,positive rate and survival rate were observed and compared.Results The relative expression levels of DK-β,Lgr5,mTOR,P70s6k in gastric cancer tissues were significantly higher than those in paracancer tissues and those in control group(P<0.05).There was a positive correlation in the expression levels of DK-β,Lgr5,mTOR,P70s6k(P<0.05).The positive expression rates of Lgr5,mTOR and P70s6k in gastric cancer tissues were obviously higher than those in paracancer tissues and those in control group,moreover the positive expression rate of DK-βin gastric cancer tissues was significantly higher than that in paracancer tissues and that in control group(P<0.05).in addition the expressions of DK-β,Lgr5,mTOR and P70s6k were not correlated with patient’s age and gender(P>0.05),moreover,there was no significant difference between the positive expression rate of DK-βand tumor’s size in patients with gastric cancer(P>0.05),however,there were significant differences in the positive rates of Lgr5,mTOR and P70s6k between the patients with tumor diameter less than 5cm(60.41%,58.33%,56.52%)and the patients with tumor diameter more than 5cm(86.48%,75.68%,72.97%)(P<0.05).In addition there were significant differences in TNM staging,histological grading and lymphatic metastasis of DK-β,Lgr5,mTOR and P70s6k(P<0.05).Conclusion There are close correlation between the expressions of DK-β,Lgr5,mTOR/P70s6k in patients with gastric cancer and tumor TNM staging,histological grade,lymph node metastases situation,in which,the high expressions or low expressions of Lgr5 and mTOR/P70s6k have a certain prediction evaluation value for patient’s prognosis,and DK-βcan be used as earlyscreening index.