9个不同产地菊茎叶中多类型资源性化学成分的分析与评价

Analysis and Evaluation of Multiple Types of Chemical Constituents in Stems and Leaves of Chrysanthemum Morifolium from Different Geographical Origins

目的:分析与评价不同产地菊茎叶中多类型资源性化学成分,为其资源化利用提供数据支撑。方法:采用紫外-可见分光光度法对不同产地菊茎叶中总黄酮、总酚、总多糖进行分析与评价;采用高效液相色谱-蒸发光散射检测法(HPLC-ELSD)对不同产地菊茎叶中单糖和寡糖组成及其含量进行分析与评价。结果:祁菊茎叶(QJ-JY)中总黄酮和总酚含量均最高,分别为(37.89±4.17)、(17.12±2.00)mg·g-1。怀白菊茎叶(HBJ-JY)和射阳杭白菊茎叶(SYHBJ-JY)中总多糖平均含量较高,分别高达(70.84±1.08)、(70.32±2.90)mg·g-1。菊茎叶中含有的单糖类成分主要是果糖和葡萄糖,双糖主要是蔗糖。其中果糖是菊茎叶中主要的糖类成分。福白菊茎叶(FBJ-JY)中果糖质量分数最高,为(94.22±6.20)mg·g-1。相关性分析显示,总黄酮与总酚呈显著正相关性,中性多糖与总黄酮和总酚均呈负相关性,可溶性糖类成分之间均呈较强正相关性。结论:菊茎叶中含有丰富的总黄酮、总酚、可溶性总多糖、果糖和葡萄糖等资源性化学成分,且不同产地之间各类型成分的含量差异较为显著。研究结果为菊茎叶的精细化利用与产业化开发提供了科学依据。

Objective:In order to analyze the multiple types of chemical constituents in the stems and leaves of Chrysanthemum morifolium from different geographical origins,and to provide the data support for their resource utilization.Methods:UV-Vis spectrophotometry was used for determining the total contents of flavonoids,phenols,and polysaccharides,and HPLC-ELSD method was used for analyzing monosaccharides and oligosaccharides in the stems and leaves of C.morifolium collected from 9 geographical origins.Results:The stems and leaves of C.morifolium from Anguo city,Hebei province(QJ-JY)had the highest content of total flavonoids and total phenols with the average content of(37.89±4.17)mg·g-1 and(17.12±2.00)mg·g-1 respectively.Compared with other geographical origins,the contents of total soluble polysaccharides were higher in the stems and leaves of C.morifolium from Zhengzhou city,Henan province(HBJ-JY)and Sheyang county,Jiangsu province(SYHBJ-JY)with the average content of(70.84±1.08)mg·g-1 and(70.32±2.90)mg·g-1 respectively.The monosaccharides contained in the stems and leaves of C.morifolium were mainly fructose and glucose,and the disaccharides were mainly sucrose.Among them,fructose was the main saccharides in the stems and leaves of C.morifolium.The stems and leaves of C.morifolium from Futianhe town,Macheng city(FBJ-JY)had the highest content of fructose with(94.22±6.20)mg·g-1.The result of correlation analysis showed that total flavonoids was positively correlated with total phenols,while they were negatively correlated with neutral polysaccharides.Moreover,there were a strong positive correlation between soluble carbohydrates(total polysaccharides,monosaccharides and oligosaccharides).Conclusion:The stems and leaves of C.morifolium were rich in these resourceful chemical compositions,and their contents were various in different geographical origins of this plant.The results provided a scientific basis for the utilization and industrial development of the stems and leaves of C.morifolium.