单羧酸转运蛋白4在结直肠癌中的表达及临床意义

The expression and the clinical significance of monocarboxylate transporter 4 in colorectal cancer

目的探讨单羧酸转运蛋白(MCTs)在结直肠癌(CRC)中的表达及临床意义。方法GEPIA2在线工具分析MCT1~4在CRC组织的表达及临床意义。选择江苏省徐州市中心医院2019年CRC组织标本存档蜡块53例,免疫组化检测MCT4蛋白的表达,正电子发射计算机断层成像(PET/CT)结果由患者术前检查获得。结果癌症和肿瘤基因图谱(TCGA)数据库中,仅有MCT4 mRNA在结肠腺癌(COAD)组织(275例)和直肠腺癌(READ)组织(92例)中的表达显著高于正常组织,差异有统计学意义(P<0.05)。MCT4高表达与CRC患者较差的无病生存期(DFS)有关(P<0.05)。此外,MCT4蛋白表达在CRC组织明显高于正常组织,差异有高度统计学意义(P<0.01)。对于MCT4高表达患者,PET/CT检查的18氟脱氧葡萄糖(18F-FDG)的标准化摄取值(SUV)高于MCT4低表达患者,差异有高度统计学意义(P<0.01)。结论MCT4在CRC发生发展中具有重要作用,可望成为新的治疗靶点。

Objective To explore the expression and the clinical significance of monocarboxylic acid transporters(MCTs)in colorectal cancer(CRC).Methods The expression status and clinical significance of MCT1-4 in CRC tissues were analyzed online by GEPIA2 database.Fifty-three cases of CRC tissue specimen wax blocks of Xuzhou Central Hospital in 2019 were selected,and the expression of MCT4 protein was detected by immunohistochemistry.The results of positron emission computed tomography(PET/CT)were obtained from the patients before operation.Results In the cancer genome atlas project(TCGA)database,only the expression of MCT4 mRNA in colon adenocarcinoma(COAD)tissues(275 cases)and rectal adenocarcinoma(READ)tissues(92 cases)were significantly higher than that in normal tissues,and the differences were statistically significant(P<0.05).High expression of MCT4 was associated with poor disease-free survival(DFS)in patients with CRC(P<0.05).Moreover,the expression of MCT4 protein in CRC tissue was significantly higher than that in normal tissue,and the difference was highly statistically significant(P<0.01).For patients with high expression of MCT4,the standardized uptake value(SUV)of 18-fluorodeoxyglucose(18F-FDG)on PET/CT was higher than that of low expression of MCT4,and the difference was highly statistically significant(P<0.01).Conclusion MCT4 plays an important role in the development of CRC and is expected to become a new therapeutic target.