吸入氢气对重症急性胰腺炎大鼠肺损伤的影响

Effect of hydrogen inhalation on lung injury induced by severe acute pancreatitis in rats

目的探讨吸入氢气(H2)对重症急性胰腺炎(SAP)大鼠肺损伤的影响。方法选取健康雄性SPF级Wistar大鼠48只,采用随机数字表法分为四组(每组12只):假手术组(SH组)、重症胰腺炎组(SAP组)、1%H2组、2%H2组。除SH组,各组通过逆行胰胆管注射5%牛磺胆酸钠的方法制作大鼠SAP模型。1%H2组和2%H2组在模型制作过程中及造模成功后24 h持续吸入相应浓度的H2。造模后24 h采集股动脉血行血气分析并计算氧合指数(PaO2/FiO2),随后处死大鼠测定肺湿干重比(W/D)并进行肺组织损伤评分,酶联免疫吸附法测定肺组织中8-羟基脱氧鸟苷(8-OHdG)、Toll样受体4(TLR-4)、核因子-κB(NF-κB)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、IL-6水平。结果与SH组比较,SAP组、l%H2组、2%H2组的W/D比值、肺组织损伤评分、8-OHdG、TLR-4、NF-κB、TNF-α、IL-1β、IL-6显著升高,PaO2/FiO2降低(P<0.05)。与SAP组比较,1%H2组、2%H2组的W/D比值、肺组织损伤评分、8-OHdG、TLR-4、NF-κB、TNF-α、IL-1β、IL-6显著降低,PaO2/FiO2升高(P<0.05)。1%H2组与2%H2组上述各指标比较,差异无统计学意义(P>0.05)。结论吸入H2能减轻重症急性胰腺炎所致肺损伤,其机制可能与抑制炎性反应和氧化应激有关。

Objective To investigate the effect of hydrogen(H2)inhalation on lung injury in rats with severe acute pancreatitis(SAP).Methods Forty-eight healthy male SPF Wistar rats were selected,four groups(12 in each group)were divided into 4 groups by random number table method,sham operation group(SH group),severe pancreatitis group(SAP group),1%H2 group,2%H2 group.With the exception of SH group,the rat SAP model was made by injecting 5%sodium taurocholate into each group through retrograde cholangiopancreatine.H2 group 1%and H2 group 2%continuously inhaled H2 at the corresponding concentration during the process of model making and 24 h after successful model making.Blood gas analysis and oxygenation index(PaO2/FiO2)were calculated after 24 h of femoral arterial blood collection after modeling.The rats were then sacrificed to determine the lung wet-to-dry weight ratio(W/D)and the lung tissue injury score.The enzyme linked immunosorbent method was used for determination of levels of 8-hydroxyl deoxyguanosine(8-OHdG),toll-like receptor 4(TLR-4),nuclear factor-κB(NF-κB),tumor necrosis factor-α(TNF-α),interleukin 1β(IL-1β),IL-6 in the lung tissues.Results Compared with SH group,the W/D ratio of SAP group,1%H2 group,1%H2 group were analyzed,the lung tissue damage rating,8-OHdG,TLR-4,NF-κB,TNF-α,IL-1β,IL-6 and IL-1 increased significantly,PaO2/FiO2 decreased(P<0.05).Compared with SAP group,W/D ratio,lung tissue damage rating,8-OHdG,TLR-4,NF-κB,TNF-α,IL-1β,IL-6 and IL-1 in 1%H2 group and 2%H2 group significantly reduced,PaO2/FiO2 elevated(P<0.05).There was no significant difference in the above indicators between the 1%H2 group and the 2%H2 group(P>0.05).Conclusion Inhalation of H2 can reduce lung injury caused by severe acute pancreatitis,and the mechanism may be related to inhibition of inflammatory response and oxidative stress.