摘要
目的探讨吸入氢气(H2)对重症急性胰腺炎(SAP)大鼠肺损伤的影响。方法选取健康雄性SPF级Wistar大鼠48只,采用随机数字表法分为四组(每组12只):假手术组(SH组)、重症胰腺炎组(SAP组)、1%H2组、2%H2组。除SH组,各组通过逆行胰胆管注射5%牛磺胆酸钠的方法制作大鼠SAP模型。1%H2组和2%H2组在模型制作过程中及造模成功后24 h持续吸入相应浓度的H2。造模后24 h采集股动脉血行血气分析并计算氧合指数(PaO2/FiO2),随后处死大鼠测定肺湿干重比(W/D)并进行肺组织损伤评分,酶联免疫吸附法测定肺组织中8-羟基脱氧鸟苷(8-OHdG)、Toll样受体4(TLR-4)、核因子-κB(NF-κB)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、IL-6水平。结果与SH组比较,SAP组、l%H2组、2%H2组的W/D比值、肺组织损伤评分、8-OHdG、TLR-4、NF-κB、TNF-α、IL-1β、IL-6显著升高,PaO2/FiO2降低(P<0.05)。与SAP组比较,1%H2组、2%H2组的W/D比值、肺组织损伤评分、8-OHdG、TLR-4、NF-κB、TNF-α、IL-1β、IL-6显著降低,PaO2/FiO2升高(P<0.05)。1%H2组与2%H2组上述各指标比较,差异无统计学意义(P>0.05)。结论吸入H2能减轻重症急性胰腺炎所致肺损伤,其机制可能与抑制炎性反应和氧化应激有关。
Objective To investigate the effect of hydrogen(H2)inhalation on lung injury in rats with severe acute pancreatitis(SAP).Methods Forty-eight healthy male SPF Wistar rats were selected,four groups(12 in each group)were divided into 4 groups by random number table method,sham operation group(SH group),severe pancreatitis group(SAP group),1%H2 group,2%H2 group.With the exception of SH group,the rat SAP model was made by injecting 5%sodium taurocholate into each group through retrograde cholangiopancreatine.H2 group 1%and H2 group 2%continuously inhaled H2 at the corresponding concentration during the process of model making and 24 h after successful model making.Blood gas analysis and oxygenation index(PaO2/FiO2)were calculated after 24 h of femoral arterial blood collection after modeling.The rats were then sacrificed to determine the lung wet-to-dry weight ratio(W/D)and the lung tissue injury score.The enzyme linked immunosorbent method was used for determination of levels of 8-hydroxyl deoxyguanosine(8-OHdG),toll-like receptor 4(TLR-4),nuclear factor-κB(NF-κB),tumor necrosis factor-α(TNF-α),interleukin 1β(IL-1β),IL-6 in the lung tissues.Results Compared with SH group,the W/D ratio of SAP group,1%H2 group,1%H2 group were analyzed,the lung tissue damage rating,8-OHdG,TLR-4,NF-κB,TNF-α,IL-1β,IL-6 and IL-1 increased significantly,PaO2/FiO2 decreased(P<0.05).Compared with SAP group,W/D ratio,lung tissue damage rating,8-OHdG,TLR-4,NF-κB,TNF-α,IL-1β,IL-6 and IL-1 in 1%H2 group and 2%H2 group significantly reduced,PaO2/FiO2 elevated(P<0.05).There was no significant difference in the above indicators between the 1%H2 group and the 2%H2 group(P>0.05).Conclusion Inhalation of H2 can reduce lung injury caused by severe acute pancreatitis,and the mechanism may be related to inhibition of inflammatory response and oxidative stress.
作者
滕娜
孙明洁
李会
TENG Na;SUN Mingjie;LI Hui(Department of Anesthesia Operating,Qingdao Municipal Hospital,Shandong Province,Qingdao266011,China)
出处
《中国医药导报》
CAS
2020年第15期26-28,33,共4页
China Medical Herald
基金
山东省卫生计生委医药卫生科技发展计划项目(2017WS209)。
关键词
氢气
胰腺炎
肺损伤
大鼠
Hydrogen
Pancreatitis
Lung injury
Rat