基于网络药理学探讨左金丸治疗胃癌前病变机制

Exploration on the mechanism of Zuojin Pills in the treatment of gastric precancerous lesions based on network pharmacology

目的运用网络药理学方法研究左金丸治疗胃癌前病变(GPL)的可能机制。方法通过TCMSP数据库获取左金丸的活性成分,利用TCMSP、Pharmmapper获得活性成分对应的靶点,利用Gene Card获得GPL的相关靶点,取共有靶点使用String、Cytoscape构建PPI网络图,并利用David对所获靶点进行通路富集分析。结果左金丸中有41种活性成分对应636个靶点,GPL共有1352个靶点,左金丸与GPL共有靶点165个,GO生物过程富集显示左金丸与GPL共有靶点参与转录调控、药物结合、细胞凋亡等多项生物过程,KEGG富集显示共有靶点在癌症通路、PI3K-Akt通路等有明显富集。结论左金丸可以通过多靶点、多通路治疗GPL,为今后研究提供参考依据。

Objective To study possible mechnism of Zuojin Pills in the treatment of gastric precancerous lesions(GPL)using network pharmacology.Methods The active components of Zuojin Pills were obtained by TCMSP database,the corresponding targets of the active components were obtained by TCMSP and Pharmapper,the related targets of GPL were obtained by Gene Card,the common targets were obtained,the PPI network map was constructed by String and Cytoscape,and the targets were analyzed by David.Results There were 41 active components corresponding to 636 targets in Zuojin Pills,1352 targets in GPL,165 targets in Zuojin Pills and GPL.The enrichment of GO biological process showed that Zuojin Pills and GPL were involved in transcription regulation,drug binding,cell apoptosis and other biological processes.The enrichment of KEGG showed that the common targets were obviously enriched in cancer pathway,PI3K-Akt pathway,and so on.Conclusion Zuojin Pills can treat GPL through multiple targets and multiple pathways,so as to provide reference for future research.