陕西不同地区马铃薯腐烂茎线虫的分离鉴定及同源性分析

Homology Analysis and Identification of Ditylenchus destructor from Different Areas of Shaanxi Province

旨在鉴定陕西不同地区马铃薯腐烂茎线虫生物型,为其防治提供理论依据。对陕西不同地区马铃薯腐烂茎线虫进行分离,对其形态进行观察并测量特征值,通过茎线虫线粒体COⅠ通用引物、ITS区通用引物及A、B生物型的特异性引物对不同地区采集马铃薯腐烂茎线虫进行PCR检测及序列分析,利用MEGA6.0进行系统发育树构建及种群同源性分析。结果表明,陕北地区腐烂茎线虫的生物型为B型,关中地区为A型。形态特征值数据显示雌成虫体长、体宽均大于雄成虫,A型的体长、体宽均大于B型。系统发育树结果显示,陕北不同地区的B型马铃薯腐烂茎线虫聚为一支,合阳、兴平的A型马铃薯腐烂茎线虫聚为一支,与分子鉴定结果相一致。

In order to provide theoretical basis for controlling Ditylenchus destructor,we identified biotypes of D.destructor from different areas of Shaanxi.In this study,the D.destructors from different areas of Shaanxi were isolated,and their morphological characteristics were observed and measured.Then the universal primers of D.destructor mitochondrial COⅠand ribosomes ITS were used for PCR detection and sequence analysis of all the D.destructor samples from different areas of Shaanxi.In addition,the specific primers of biotype A and B were also used for PCR detection and sequence analysis of all the samples.MEGA 6.0 was used for phylogenetic tree construction and population homology analysis.The results showed that,the biotype of D.destructors from Northern Shaanxi was type B,and the biotype of D.destructors from Guanzhong area was type A.Morphological characteristic value data showed that female adults were longer and wider than male adults,and type A were longer and wider than type B in both female and male adults.Phylogenetic tree showed that type B D.destructors from Northern Shaanxi clustered in one clade,and type A D.destructors from Heyang and Xingping clustered in one clade,so it was consistent with the results of molecular identification.