诺如病毒GI.6[P11]与GII.13[P16]共感染的分子特征分析

Analysis on molecular characteristics of co-infection with GI.6[P11]and GII.13[P16]noroviruses

目的在同一个标本中检测出了两种组别的诺如病毒,对这两个诺如病毒的分子特征进行初步分析。方法利用普通RT-PCR对两个病毒的部分聚合酶区及衣壳蛋白区片段进行扩增和序列测定。通过BioEdit软件进行序列比对分析,采用MEGA软件及BEAST软件分别构建进化树进行进化分析,Simplot软件进行重组位点分析。结果序列进化分析显示标本中确实存在两种不同型别且不同组别的诺如病毒,分别为GI.6[P11]型与GII.13[P16]型;时间进化分析显示GI.P11位于2013—2018分支,GII.13位于GII.13[P16]的2013—2018分支,平均替换速率分别为1.6×10^-3及1.9×10^-3替换/位点/年;GII.13[P16]可能的重组位点在病毒的开放读码框架1上。结论本研究对少见的共感染的两种型别重组诺如病毒进行了初步分析,为同种型别的深入研究提供了基础参考数据。

Objective To analyze the molecular characteristics of the two genotypes of norovirus detected in the same sample.Methods The partial RNA-dependent RNA polymerase gene and capsid protein fragments of the two virus strains were amplified by RT-PCR and were then sequenced.Sequence alignments were performed using Bioedit software.MEGA and BEAST software were used for constructing the phylogenetic tree and the analysis.Recombinant break-points were analyzed by Simplot software.Results Phylogenetic analysis identified that there were two different genotypes and groups of noroviruses,GI.6[P11]and GII.13[P16]in the same sample.Time evolution analysis showed that GI.P11 belonged to 2013-2018 cluster.GII.13 belonged to 2013-2018 cluster of GII.13[P16].The average substitution rates were 1.6×10^-3 and 1.9×10^-3 substitutions per site per year,respectively.The possible recombinant break-point of GII.13[P16]may locate in the Open Reading Frame 1 of the virus.Conclusions In this study,two rare genotypes of recombinant norovirus in the co-infection were analyzed,providing basic reference data for further study of the same type of the virus.