广西百色壮族原发性高血压与β2肾上腺素能受体基因多态性的相关性

Correlation between primary hypertension andβ2 adrenergic receptor gene polymorphism in Baise,Guangxi

目的探讨百色地区壮族人群受试者β2-肾上腺素能受体(β2-AR)基因多态性与高血压的关系和原发性高血压的遗传学特征。方法98例高血压病例患者,患病史至少两年。同时还有109例健康的对照受试者。对所有受试者进行基本的体格检查及空腹血浆生化指标的测定,然后再使用多聚酶链反应技术(polymerase chain reaction,PCR)对DNA进行分析,以鉴定β2-AR基因rs6879202与rs2053044的位点的基因型和等位基因频率。并采用SPSS 19.0软件中回归模块中的Logistic对有显著性统计学差异的位点的基因频率诱发高血压的危险性进行了一定的探讨。结果病例组与对照组在性别比、肌酐、吸烟率、甘油三酯、尿酸等之间有统计学差异。病例组和对照组β2-AR基因rs6879202位点CC、CG、GG基因频率分别为3.10%,72.40%,24.50%;13.80%,57.80%,28.40%;两组的基因型频率分布之间有统计学意义(χ^2=8.809,P=0.012)。Logistic分析结果表明对rs6879202位点而言CG较其他两个基因频率诱发高血压的风险更大。结论百色地区壮族患者β2-AR基因rs6879202位点多态性与原发性高血压有相关性,而β2-AR基因rs2053044位点多态性与该地区该民族的原发性高血压没有相关性。

Objective To investigate the relationship betweenβ2-adrenergic receptor(β2-AR)gene polymorphism and hypertension and genetic characteristics of primary hypertension in Zhuang population in Baise district.Methods This study consisted of 98 patients,who had hypertension with a history of at least two years,and 109 healthy individuals came from the same ethnical group.For all the participants,their general physical health was examined and the biochemical biomarkers of their blood before breakfast were tested by the analytical instruments.Then,DNA was analyzed using polymerase chain reaction(PCR)in order to identify the genotypes and allele frequencies ofβ2-AR genes rs6879202 and rs2053044.Meanwhile,Logistic regression of SPSS 19.0 software was implemented to explore the risk of hypertension induced by the gene frequency of sites with significant statistical differences.Results There were significant differences in sex ratio,creatinine,smoking rate,triglyceride and uric acid between case group and control group.Forβ2-ARrs6879202 gene locus:CC,CG,GG between two groups,the frequencies were 3.10%,72.40%,24.50%;13.80%,57.80%,28.40%,respectively;the genotype frequency distributions between two groups were statistically different(χ^2=8.809,P=0.012).The results of Logistic analysis showed that for rs6879202,CG was associated with a higher risk of hypertension than the frequency of the other two genes.Conclusion There was correlation betweenβ2-AR gene rs6879202 locus polymorphism and primary hypertension in Zhuang ethical population in Baise region,while there was no correlation betweenβ2-AR gene rs2053044 locus polymorphism and primary hypertension in this region and nationality.