鲢EST-SSR标记的开发及其与生长性状关联性分析

DEVELOPMENT OF EST-SSR MARKERS AND ANALYSIS OF GROWTH TRAIT IN SILVER CARP

研究采用Illumina高通量测序技术对鲢(Hypophthalmichthys molitrix)卵组织进行转录组测序分析,筛选多态EST-SSR标记并与生长性状进行关联性分析。研究结果显示:(1)测序组装总Unigene 77129个, SSR共24458个,占所测EST数据的31.7%。鲢包含SSR的EST序列主要有二碱基、三碱基、四碱基和五碱基组成。二碱基中, AC含量最丰富;(2)随机挑选175条EST序列设计SSR引物,筛选出多态EST-SSR标记36个, Blast结果显示有10条已知基因功能;(3)全同胞家系检测结果显示有两个标记与鲢的生长性状相关。SCE26与体长和体重显著相关(P<0.05), SCE65与肥满度显著相关(P<0.05)。研究结果将为鲢的分子标记辅助育种的应用提供基础理论指导。

Silver carp is one of important breeding fish in China. It is necessary to study the growth traits markers to accelerate the molecular marker-assisted breeding techniques. The simple sequence repeat(SSR) from the transcriptome of egg in silver carp were characterized by high-throughput Illumina sequencing, and genotypes associated growth traits were analyzed. The main results are as follows:(1) 77129 unigenes were detected by assembly, 31.7% of 24458 ESTs contained repeat motifs of various types(dinucleotide, trinucleotide, tetranucleotide and pentanucleotide) and lengths with AC being the most abundant dinucleotides one.(2) 175 EST-SSR primers were randomly characterized and 36 polymorphic EST-SSR were developed in silver carp. The results of Blast indicated that 10 EST-SSR genes are known.(3) The results showed that two loci were significantly correlated with growth traits in full-sibling family in silver carp.One locus SCE26 was significantly(P<0.05) correlated with body length and body weight, and SCE65 was significantly(P<0.05) correlated with condition factor. This study will provide theoretical basis and technical methods for the development of the marker-assisted breeding system in silver carp.