基于代谢组学的绝经后骨质疏松症实性证素研究

Study on Syndrome of Postmenopausal Osteoporosis Based on Metabolomics

目的运用代谢组学研究方法研究绝经后骨质疏松症常见实性证素与其代谢产物之间的关系。方法通过证素辨证的方法选取绝经后骨质疏松症的常见实性证素:肾虚对照组、肾虚血瘀组、肾虚痰湿组、肾虚气滞组,应用核磁共振氢谱(H-NMR)技术测定不同证型之间的血清代谢产物,并通过多元分析代谢谱之间的差异。结果绝经后骨质疏松症的肾虚组与肾虚气滞组、肾虚组与肾虚痰湿组、肾虚气滞组与肾虚血瘀组、肾虚气滞组与肾虚痰湿组、肾虚血瘀组与肾虚与痰湿组5组之间的血清代谢成分存在明显的差异。结论各组间的差异代谢产物对能量代谢、氨基酸代谢等有不同的影响,相对应在绝经后骨质疏松症的实性证素之间有不同的表达,对于研究中医证素与代谢组学之间的关系提供了依据。

Objective Using metabolomics research method,the relationship between the common sex hormones and their metabolites in postmenopausal osteoporosis( PMOP) was studied. Methods The common solid syndrome elements of PMOP were selected by syndrome differentiation: kidney deficiency control group,kidney deficiency and blood stasis group,kidney deficiency and phlegm dampness group,kidney deficiency and Qi stagnation group. The serum metabolites between different syndromes were determined by H-NMR and the differences between metabolic profiles were analyzed by multivariate analysis. Results In kidney deficiency group and Qi stagnation group,kidney deficiency group and kidney and phlegm dampness group,kidney deficiency and Qi stagnation group and kidney deficiency and blood stasis group,kidney deficiency and Qi stagnation group and kidney deficiency and phlegm dampness group,kidney deficiency and blood stasis group and kidney deficiency and phlegm dampness group,there were significant differences of serum metabolic components. Conclusion Different metabolites in different groups have different effects on energy metabolism and amino acid metabolism. Correspondingly,there are different expressions of real sex syndrome elements in PMOP. It provides a basis for studying the relationship between TCM syndrome elements and metabolomics.