基于GC/MS的皮肤T细胞淋巴瘤(CTCL)异种移植小鼠血浆和皮肤组织的代谢组学分析

Metabolomic analysis of cutaneous T-cell lymphoma(CTCL) xenograft mice plasma and skin tissue based on GC/MS

目的探讨皮肤T细胞淋巴瘤(cutaneous T-cell lymphoma,CTCL)异种移植小鼠血浆和皮肤组织代谢组的变化,寻找与其相关的差异代谢物。方法将21只Balb/c雌性裸鼠分为实验组和对照组,利用HH细胞(T细胞淋巴瘤ATCC CRL-2105)造模,基于GC/MS对CTCL荷瘤鼠的血浆和肿瘤组织样品进行代谢组学分析。结果共有62种差异代谢物,其中13种在血浆和组织样品中均有出现,结合课题组之前基于LC/MS的CTCL荷瘤鼠血浆和肿瘤组织的代谢组学的研究结果,有9种差异代谢物在GC/MS和LC/MS分析方法中同时出现。血浆中支链氨基酸和葡萄糖升高,血浆和肿瘤组织中3-磷酸甘油升高,血浆中鞘氨醇-1-磷酸盐降解的o-磷酸乙醇胺升高。结论62种差异代谢物可以提高对CTCL代谢物及其相关通路的认识,有助于CTCL的临床诊断。

Objective To investigate the changes in plasma and skin tissue metabolomes in mice after cutaneous T-cell lymphoma(CTCL),and to find differential metabolites associated with them.Methods A total of 21 Balb/c female nude mice were randomly divided into control group and experimental group.CTCL model was induced by HH cell(T cell Lymphoma ATCC CRL-2105).Plasma and tumor tissue samples of cutaneous T-cell lymphoma mice were analyzed by untargeted metabolomics method based on GC/MS and principal component analysis was performed.Results A total of 62 differential metabolites were identified,13 of which were found in both plasma and tissue samples.Combined with our previous metabolomics studies on LC/MS-based CTCL tumor-bearing mice plasma and tumor tissue,9 identical differential metabolisms were identified by GC/MS and LC/MS platforms.The branched-chain amino acids and glucose were increased in plasma,glycerol-3-phosphate was increased in plasma and tumor tissues,and o-phosphoethanolamine degraded by sphingosine-1-phosphate was increased in plasma.Conclusion Sixty-two differential metabolites have improved the understanding of CTCL metabolites and related pathways,which is helpful for the clinical diagnosis of CTCL.