LncRNA NR2F2-AS1靶向抑制miR-129-5p调控胶质瘤细胞增殖和凋亡的机制研究

LncRNA NR2F2-AS1 regulates proliferation and apoptosis of the glioma cells by targeting miR-129-5p

目的研究lncRNA NR2F2-AS1和miR-129-5p在胶质瘤中的表达及其对胶质瘤U251细胞增殖和凋亡的影响。方法qRT-PCR检测lncRNANR2F2-AS1和miR-129-5p在胶质瘤组织和细胞中表达水平,MTT法和流式细胞术检测U251细胞增殖和凋亡,Westernblot检测细胞中CyclinD1、p21、Bax和Bcl-2的蛋白表达水平,双荧光素酶报告系统验证NR2F2-AS1和miR-129-5p的调控关系。结果与正常脑组织相比,在胶质瘤组织中lncRNA NR2F2-AS1的含量显著升高(P<0.05),miR-129-5p的含量则显著下降(P<0.05);干扰NR2F2-AS1表达和过表达miR-129-5p均可抑制胶质瘤U251细胞增殖并促进细胞凋亡,使细胞中CyclinD1和Bcl-2含量降低(P<0.05),p21和Bax含量升高(P<0.05);lncRNA NR2F2-AS1靶向负调控miR-129-5p的表达;抑制miR-129-5p表达逆转了干扰NR2F2-AS1表达对U251细胞增殖、凋亡的作用。结论干扰lnc RNANR2F2-AS1通过靶向促进miR-129-5p表达抑制胶质瘤U251细胞增殖,诱导细胞凋亡。LncRNA NR2F2-AS1可能是胶质瘤的分子靶点。

Objective To investigate the expression of lncRNA NR2F2-AS1 and miR-129-5p in gliomas and their effects on the proliferation and apoptosis of U251 cells.Method Expression levels of lncRNA NR2F2-AS1 and miR-129-5p were measured by quantitative real-time polymerase chain reaction(qRT-PCR).The proliferation and apoptosis of U251 cells were determined by MTT assay and flow cytometry,respectively.Expression of CyclinD1,p21,Bax and Bcl-2 were detected by Western blot.Dual-luciferase reporting assay system was performed to confirm the targeted relationship between lncRNA NR2F2-AS1 and miR-129-5p.Results Compared with normal brain tissue,the level of lncRNA NR2F2-AS1 in glioma tissues was increased significantly(P<0.05),and the level of miR-129-5p was decreased remarkably(P<0.05).Interfering with the expression of NR2F2-AS1 and over-expression of miR-129-5p inhibited the proliferation and promoted the apoptosis of glioma U251 cells,and reduced the expression levels of CyclinD1 and Bcl-2 in the cells(P<0.05),while increased the content of p21 and Bax(P<0.05).LncRNA NR2F2-AS1 targets and negatively regulates the expression of miR-129-5 p.Inhibition of mi R-129-5p reversed the effects of interfering NR2F2-AS1 on the proliferation and apoptosis of U251 cells.Conclusion Interfering lncRNA NR2F2-AS1 inhibits the proliferation and induces apoptosis of glioma U251 cells by targeting and promoting the expression of miR-129-5p.LncRNA NR2F2-AS1 may be a molecular target of glioma.