外源NO对PEG胁迫下燕麦幼苗生长及生理特性的影响

Effects of Exogenous Nitric Oxide on the Growth and Physiological Characteristics of Oat Seedlings under PEG Stress

为探讨外源一氧化氮(NO)对燕麦幼苗生长和抗旱性的影响,选用高寒皮燕麦品种青燕1号为材料,以15%PEG-6000模拟干旱胁迫,研究了添加0.2 mmol·L^-1外源NO后其幼苗生长及抗氧化酶等生理生化特性的变化。结果表明,PEG胁迫显著抑制了供试材料的生长,降低了其叶片相对含水量及叶绿素含量等。添加外源NO可以有效缓解干旱胁迫对供试燕麦幼苗的伤害,提高叶片保护酶(SOD)活性,降低超氧阴离子(O2^·-)、过氧化氢(H2O2)和丙二醛(MDA)含量,促进了脯氨酸(Pro)和可溶性糖(SSC)的积累,维持了较高的渗透调节能力,增强了其抗旱性。

In order to investigate the effects of exogenous nitric oxide(NO) on the growth and drought resistance of Alpine oats variety Qingyan 1 seedlings, the effects of 0.2 mmol·L^-1 exogenous NO on the growth of seedlings and the activities of antioxidant enzymes were studied by using 15% PEG-6000 to simulate drought stress. The results showed that PEG stress significantly inhibited the growth of the tested material and reduced the relative water content and chlorophyll content of the leaves. And the addition of exogenous NO could effectively alleviate the damage of drought stress to oat seedlings, increase the activity of SOD in leaves, reduce the content of superoxide anion(O2^·-), hydrogen peroxide(H2O2) and malondialdehyde(MDA), promote the accumulation of proline(Pro) and soluble sugar(SSC), maintain a high osmotic regulation ability, and enhance its drought resistance.