SNHG3基因在子宫腺肌病中的表达及对于治疗方向的初步研究

Preliminary study on SNHG3 gene expression and its therapeutic role in adenomyosis

目的:探究小核仁RNA宿主基因3(SNHG3)在子宫腺肌病中的表达及意义。方法:收集32例子宫腺肌病患者的异位子宫内膜和45例对照组的子宫内膜组织,进行qRT-PCR实验,检测SNHG3基因的mRNA表达水平;采用Western blot技术检测SNHG3基因的蛋白表达水平。同时收集这些病人的诊疗数据,分析两组患者临床性状的差异性。结果:SNHG3基因在子宫腺肌病异位内膜组织中mRNA表达比对照组增高,其表达差异具有统计学意义(t=3.190,P=0.002)。腺肌病组的SNHG3基因的蛋白表达水平较对照组上调,差异有统计学意义(t=15.169,P=0.001)。两组临床资料结果显示是否合并子宫肌瘤在两组间差异具有统计学意义(P=0.002)。结论:SNHG3基因与子宫腺肌病的发生具有一定的相关性,为治疗方向提供新思路。

Objective:To investigate the expression and significance of the SNHG3 gene in adenomyosis.Methods:Ectopic endometria were obtained from 32 patients with adenomyosis and 45 controls,and subjected to qRT-PCR determination for the mRNA expression level in SNHG3 gene.The protein level of SNHG3 gene was also detected by Western blot.Diagnosis and treatment information were collected form the patients described above,and analyzed for difference by clinical traits between the two groups.Results:The mRNA in SNHG3 gene was highly expressed,and protein level of SNHG3 gene was up-regulated in endometria of adenomyosis compared to the control group(t=3.190,P=0.002;t=15.169,P=0.001,respectively).The clinical data indicated statistical difference between groups regarding presence or absence of concomitant with adenomyosis(P=0.002).Conclusion:SNHG3 gene has certain correlation with the occurrence of adenomyosis.The findings may provide a new concept for treatment of this condition.