新疆地区胃食管反流病食管外表现与白细胞介素1β基因多态性的相关性

Correlation between extra-esophageal manifestations and interleukin-1β gene polymorphism in patients with gastroesophageal reflux disease in Xinjiang region

目的探索IL-1β基因多态性与新疆地区胃食管反流病(gastroesophageal reflux disease,GERD)患者食管外表现的相关性。方法持续纳入2018年1月至2019年4月新疆维吾尔自治区人民医院确诊GERD患者1100例,搜集患者一般信息和饮食习惯,并根据是否发生反流性咽喉炎、反流性咳嗽与反流性哮喘分为食管外表现组和无食管外表现组。提取胃黏膜组织DNA并通过聚合酶链式反应产物直接测序法检测IL-1β-511位点基因多态性,并采用Logistic回归分析其与食管外表现的相关性。结果新疆地区GERD患者反流性咽喉炎、反流性咳嗽和反流性哮喘的发生率分别为26.91%、16.09%和12.27%。两组患者在IL-1β-511位点基因型分布差异有统计学意义(χ~2=22.567,P<0.001)。在校正饮食因素和民族差异后,G等位基因的突变是食管外表现(反流性咽喉炎、咳嗽、哮喘)的保护因素,显性模型证实AG与GG基因型GERD患者发生食管外表现症状危险度降低。结论IL-1β-511位点基因多态性与新疆地区GERD患者食管外表现存在明显相关性。

Objective To explore the association between IL-1β gene polymorphism and extra-esophageal manifestations in patients with gastroesophageal reflux disease(GERD) in Xinjiang. Methods 1 100 patients with GERD in People’s Hospital of Xinjiang Uygur Autonomous Region from Jan. 2018 to Apr. 2019 were continuously enrolled. General information and dietary habits of the patients were collected. According to whether reflux pharyngitis, reflux cough and reflux asthma presence, subjects were divided into the esophageal manifestation group and the non-esophageal manifestation group. The DNA of gastric mucosa was extracted and the polymorphism of IL-1β-511 gene was detected by polymerase chain reaction. The correlation between the expression of IL-1β-511 gene and the expression of extra-esophageal manifestations was analyzed by Logistic regression.Results The incidence of reflux pharyngitis, reflux cough and reflux asthma were 26.91%, 16.09% and 12.27%, respectively in patients with GERD in Xinjiang region. There was a statistically difference in genotype distribution at the IL-1β-511 locus between the two groups(χ~2=22.567, P<0.001). After adjusting for dietary and ethnic differences, the G allele mutation was a protective factor for extra-esophageal manifestations(reflux pharyngitis, cough, asthma), and dominant model confirms the risk of esophageal symptoms in GERD patients with AG and GG was declined. Conclusion IL-1β-511 polymorphism is associated with extra-esophageal manifestations in patients with GERD in Xinjiang region.