超声波对樟芝孢子萌发与细胞形态的影响

Effects of Ultrasonic Treatment on the Germination and Cellular Morphology of Antrodia camphorara Arthrospores

以樟芝菌节孢子为研究对象,通过单因素试验,分析超声介入时间点、超声功率密度、超声频率和超声时长对樟芝菌节孢子发芽率的影响,优化了超声波对樟芝菌节孢子萌发的最佳处理条件:在樟芝菌节孢子接种到培养基中培养9 h后,超声波频率为22 kHz、功率密度为60 W/L处理15 min。采用扫描电镜观察了超声波对节孢子细胞壁的影响,发现超声波处理引起樟芝菌节孢子细胞壁表面形成很多针状物,在一定程度上破坏了细胞壁的结构,随着恢复培养时间的推移,逐渐恢复为表面光滑的细胞壁,这些结果表明可能是由于超声波处理在一定程度上破坏了樟芝节孢子细胞壁的结构,从而促进了樟芝菌节孢子的萌发。

Taking Antrodia camphorara arthrospores as the research object,the effects of ultrasonic intervention time point,ultrasonic power density,ultrasonic frequency and ultrasonic time on the germination rate of Antrodia camphorara arthrospores were analyzed by a single factor test.The optimal treatment conditions for germination of Antrodia camphorara arthrospores by ultrasound were optimized:after arthrospores of Antrodia camphorara cultivated for 9 hours in medium culture,ultrasonic treatment were performed at power density of 60 w/L and frequency of 22 kHz for 15 min.Using scanning electron microscopy(SEM)to observe the effects of the ultrasonic on the cell walls of arthrospores,it was found that the ultrasonic treatment causes the cell walls of arthrospores formed many needles,which destroyed the structure of the cell wall to a certain extent.With the restoration of culture time,it gradually returned to a smooth cell wall.These results suggested that the ultrasonic treatment promoted the germination of arthrospores likely due to the destroyed cell call caused by ultrasonic treatment.