大黄素下调牙周组织细胞核因子-κB是治疗中、重度慢性牙周炎机制之一

Down-regulation of NF-κB in periodontal tissue cells is one of the mechanisms of emodin in the treatment of moderate and severe chronic periodontitis

目的:探讨大黄素治疗中、重度慢性牙周炎的可能机制。方法:将102例中、重度慢性牙周炎患者均等分为对照组和观察组(各51例)。患者经牙周基础治疗,观察组患者加服大黄素,早晚各1片(1.55 mg/片),连服7~14 d。治疗前及治疗后3个月检测牙龈出血指数(BI)、临床附着丧失(CAL)、探诊深度(PD);计算总有效率及健康状况评分(SF-36)评分。在龈下刮治术和术后14 d时分别取牙周组织行细胞培养(2组各12例),检测细胞中过氧化物酶体增殖物激活受体-γ(PPAR-γ)表达、牙周组织细胞核因子-κB(NF-κB)、IL-1β、IL-4及IL-6水平。结果:与治疗前和对照组比较,观察组BI、CAL、PD均明显降低(P<0.05);总有效率及SF-36评分高于对照组(P<0.05)。观察组牙周组织细胞PPAR-γ高表达(P<0.05)、NF-κB低表达(P<0.05),IL-1β、IL-4及IL-6水平降低(P<0.05)。结论:大黄素通过下调牙周组织细胞中NF-κB水平是有效治疗中、重度慢性牙周炎的机制之一。

Objective:To study the mechanism of emodin in the treatment of moderate and severe chronic periodontitis.Methods:102 patients with moderate and severe chronic periodontitis were divided into 2 groups(n=51).All the patients were treated with periodontal basic therapy,and those of the observation group was then treated with emodin(1.55 mg),2/d for 7-14 days.BI,CAL and PD were measured before and 3 months after treatment.Periodontal tissue cells were cultured(n=12),PPAR-γ,NF-κB,IL-1β,IL-4 and IL-6 expression of the cells were measured immedietly after and 14 days after basic periodontal trerapy.The effective rate and SF-36 score were analyzed.Results:Compared with the pre-treatment and the control group,in the observation group BI,CAL and PD were decreased(P<0.05),the scores of total effective rate and quality of life(SF-36)were higher(P<0.05),the expression of NF-κB,IL-1β,IL-4 and IL-6 decreased(P<0.05),PPAR-γincreased(P<0.05).Conclusion:Inhibition of NF-κB signaling pathway is one of the mechanisms of emodin in the treatment of moderate and severe clronic periodotitis.