摘要
本试验对山东省内不同地区番茄溃疡病发病情况进行调查,采集具有典型症状的病样,采用组织分离方法分离纯化病原菌,并对其进行形态学观察、分子生物学鉴定和致病性测定。结果表明,不同地区番茄病样分离得到的4个菌株均属于溃疡病菌Cmm,但致病力存在差异。本研究可为番茄溃疡病的准确鉴定和病害防治提供参考。
In this experiment,the incidence of tomato bacterial canker disease was investigated in different areas of Shandong Province.The diseased samples with classical symptom were collected,and then the pathogen was isolated by tissue-isolation method and the morphological characteristics,molecular identification and pathogenicity test were conducted.The results showed that four strains were obtained from the investigated areas in Shandong Province.All they belonged to Cmm with different pathogenicity.This research could provide references for the identification and control of tomato bacterial canker.
作者
苏晓梅
刘淑梅
李嘉琦
王施慧
侯丽霞
Su Xiaomei;Liu Shumei;Li Jiaqi;Wang Shihui;Hou Lixia(Institute of Vegetables and Flowers,Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory for Biology of Greenhouse Vegetables/Shandong Branch of National Improvement Center for Vegetables/Huang-Huai-Hai Region Scientific Observation and Experimental Station of Vegetables,Ministry of Agriculture and Rural Affairs(Shandong),Jinan 250100,China;College of Horticulture,Jilin Agricultural University,Changchun 130118,China)
出处
《山东农业科学》
2020年第5期100-104,共5页
Shandong Agricultural Sciences
基金
山东省农业良种工程项目(2017LZGC013)
山东省农业科学院农业科技创新工程项目(CXGC2018E08)
国家现代农业产业技术体系建设专项资金项目(CARS-23-G14)。
关键词
番茄溃疡病
CMM
病原菌
分离鉴定
Bacterial canker of tomato
Cmm
Pathogen
Isolation and identification