铁皮石斛花对亲代及子代大鼠睾丸组织和精子质量的影响

Effects of Dendrobium officinale flowers on testivular tissue and sperm quality in parent and offspring rats

目的研究铁皮石斛花对亲代及子代雄性大鼠睾丸、附睾和精子数量、质量的影响,为评价铁皮石斛花安全性提供依据。方法设低、中、高3个剂量组和对照组,每组10只SD雄性大鼠,各剂量组以喂饲方式给予含2.0、4.0、6.4 g/kgbw铁皮石斛花的基础饲料。喂饲3个月后测定亲代(P)及子一代(F1)和子二代(F2)大鼠体重、睾丸、附睾脏器重并计算脏体比;显微镜下观察精子数量、活动率和畸形率;采用苏木精-伊红染色法观察睾丸、附睾组织的组织学形态。结果各组P、F1和F2雄性大鼠体重、睾丸重、睾丸体比、附睾重、附睾体比、精子数量和精子活动率比较,差异均无统计学意义(P>0.05)。高剂量组P、F1、F2雄性大鼠精子畸形率比较,差异均无统计学意义(P>0.05)。P、F1和F2雄性大鼠睾丸和附睾组织形态均无明显的改变。结论铁皮石斛花对亲代及子代雄性大鼠睾丸组织和精子数量、质量均未见明显不良影响。

Objective To learn the effects of Dendrobium officinale flowers on testivular tissue morphology and sperm quality in parent and offspring rats,so as to provide reference for safety evaluation of Dendrobium officinale flowers.Methods The 40 SD rats was randomly divided into the low-,middle-,high-dose and the control group,given 2.0,4.0,6.4 and 0 g/kgbw Dendrobium officinale flowers,respectively.After three months,the body weight,mass and organ/body coefficients of testis and epididymis of parent(P)and offspring(F1,F2)rats were measured;the number,activity and deformity of sperms were observed under microscope;the changes of testis and epididymis were observed by hematoxylin eosin staining.Results There were no significantly statistical differences in the body weight,mass and organ/body coefficients of testis and epididymis,sperm quantity,sperm motility rate among four groups of P、F1、F2 male rats(P>0.05).There were no significantly statistical differences in sperm malformation rate between the high-dose group and the control group(P>0.05).There was no significant change in testis and epididymis of P,F1 and F2 male rats.Conclusion Dendrobium officinale flowers did not show obviously adverse effects on testivular tissue morphology and sperm quality in parent and offsping rats.