广州番禺南沙地区无偿献血人群HTLV感染情况调查

Investigation and analysis of HTLV infection in blood donors in Panyu and Nansha District of Guangzhou

目的了解珠三角局部沿海地区献血人群人类T淋巴细胞白血病病毒(HTLV)的感染状况,为国家卫生行政部门今后制定地区性血液安全政策提供参考依据。方法对本站无偿献血标本,采用酶联免疫双抗原夹心方法进行初筛,初筛阳性的标本进行双孔复查,对复查阳性的标本进行化学发光法检测,化学发光法阳性标本进行蛋白印迹法(WB)和病毒核酸检测法(PCR)确证。结果2016年3月-2019年3月共筛查92047例无偿献血样本(未剔除重复献血者样本),发现酶免初筛阳性标本34例,其中化学发光阳性5例(不存在酶免及化学发光重复阳性病例),蛋白印迹法及病毒核酸检测均阴性。结论广州市番禺及南沙地区无偿献血人群是HTLV感染非流行区,综合考虑血液安全和经济成本效益,降低HTLV经血传播风险,血液可采用滤白处理。

Objective To investigate the infection status of human T-lymphocytic leukemia virus(HTLV)in blood donors in the coastal areas of the Pearl River Delta,and provide reference for the national health administrative department to formulate regional blood safety policies in the future.Methods The blood donors′samples were screened by enzyme-linked immunosorbent double-antibody sandwich method,and the positive samples in screening were subjected to double-hole re-examination,and the positive-reviewed samples were detected by chemiluminescence method,and the chemiluminescence positive samples were subjected to Western Blotting(WB)and viral nucleic acid detection(PCR)confirmation.Results From March 2016 to March 2019,a total of 92047 donor samples were screened(samples of repeated blood donors were not excluded).34 ELISA-positive cases were found in initial screening,of which 5 cases were positive for chemiluminescence,and negative for Western blot and viral nucleic acid tests.Conclusion Voluntary blood donors in Panyu and Nansha areas of Guangzhou is a low-endemic population for HTLV infection.Considering blood safety and economic benefits,leukocyte-depleted blood components can be promoted to reduce the risk of HTLV transfusion.