高压氧联合湿性愈合治疗大鼠皮肤慢性难愈合溃疡的疗效研究

Hyperbaric oxygen improves the effectiveness of wet healing in the treatment of refractory skin ulcers

目的 观察高压氧联合湿性愈合治疗大鼠皮肤慢性难愈合溃疡的疗效。方法 将60只雄性SD大鼠分为普通治疗组、湿性愈合组、联合治疗组,每组20只。大鼠常规饲养1周后造模,造模前6 h禁食,制成慢性皮肤溃疡模型。普通治疗组大鼠按一般性皮肤溃疡治疗,湿性愈合组大鼠按湿性愈合治疗,联合治疗组大鼠在湿性愈合治疗基础上,辅以6个疗程的高压氧治疗。治疗2个疗程、4个疗程、6个疗程时,计算各组大鼠的创面愈合率,采用组织病理学分析对大鼠皮肤创面的恢复情况进行评定。结果 与普通治疗组同时间点比较,湿性愈合组和联合治疗组大鼠2个疗程、4个疗程、6个疗程的创面愈合率均较高(P<0.05)。与湿性愈合组同时间点比较,联合治疗组大鼠2个疗程[(51.0±8.7)%]、4个疗程[(75.0±5.6)%]、6个疗程[(88.0±4.4)%]的创面愈合率均较高(P<0.05)。与普通治疗组比较,湿性愈合组和联合治疗组大鼠组织病理学评分较优异(P<0.05)。与湿性愈合组比较,联合治疗组大鼠组织病理学评分较优异(P<0.05)。结论 单一湿性愈合治疗慢性皮肤溃疡的疗效优于普通治疗,高压氧联合湿性愈合治疗慢性皮肤溃疡的疗效更为优异。

Objective To explore the effect of combining hyperbaric oxygen inhalation with wet healing in the treatment of chronic refractory ulcers of the skin.Methods Sixty male Sprague-Dawley rats were randomly formed into a general treatment group(group A),a wet healing group(group B),and a hyperbaric oxygen+wet healing group(group C),each of 20.Chronic and refractory skin ulcers were induced on all of the rats after one week of routine feeding and 6 hours of fasting.Six courses(once daily for 10 days/course)of the three treatments were then administered.After 2,4 and 6 courses of treatment the wound healing rate and recovery were analyzed using his-topathological methods.Results After 2,4 and 6 courses of treatment,the average wound healing rates of groups B and C were significantly higher than group A′s average,and that of group C was significantly better than that of group B.After 6 courses of treatment,group C′s average histopathology score was significantly higher than that of group B,and those of groups B and C were both significantly higher than group A′s average.Conclusion Single wet healing is better than common treatment for chronic skin ulcers,but hyperbaric oxygen inhalation can further improve its effectiveness.