红豆杉提取物对慢性阻塞性肺疾病小鼠的药效学评价

Pharmacodynamics Evaluation of Taxus Chinensis Extract on Chronic Obstructive Pulmonary Disease Mice

目的观察红豆杉提取物灌胃给药对吸烟致慢性阻塞性肺病(COPD)模型小鼠的影响,对其药效进行评价,并对其作用机制进行初步探讨。方法 C57BL/6小鼠烟熏3个月后,收集肺泡灌洗液(BALF)和肺组织,对BALF进行白细胞分类计数,取肺组织做组织切片进行HE染色和AB/PAS染色,观察炎症细胞浸润情况,并对超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、髓过氧化物酶(MPO)活性和TNF-α、KC、MIP-2表达量进行测定。结果红豆杉提取物具有降低白细胞总数的作用,可显著增加SOD、GSH含量,抑制MPO活性,且具有显著抑制TNF-α、KC炎症因子的作用。结论红豆杉提取物对COPD动物模型有一定的治疗效果,其作用机制可能与抑制中性粒细胞迁移、抑制炎症细胞因子的产生、抑制黏液分泌有一定关系。

OBJECTIVE To observe the effect of Taxus chinensis extract on chronic obstructive pulmonary disease(COPD) mice, evaluate its efficacy, and study its mechanisms. METHODS Smoke mice model for COPD was established, and the broncho alveolar lavage fluid(BALF) and lung tissues were finally collected for total differential white blood cells counting and HE and AB/PAS staining. The SOD, GSH, MPO activity, as well as TNF-α, KC and MIP-2 expression were determined to evaluate inflammatory infiltration. RESULTS The extract of Taxus chinensis significantly decreased the content of WBC, increased the activity of SOD and GSH, and inhibited MPO activity and TNF-α and KC inflammatory factors. CONCLUSION The extract of Taxus chinensis show effectiveness on COPD mice model, with its mechanism of action associated with inhibition of migration of neutrophil, the generation of inflammatory cytokines and mucus secretion of airway.