含色散和电穿孔效应的球形细胞在纳秒脉冲下的生物电效应仿真

Simulation of Bioelectric Effects of Dispersive Electroporated Spherical Cell with Nanosecond Pulsed Electric Field

为准确研究纳秒脉冲(nanosecond pulses electric field,ns PEF)作用下细胞跨膜电位的分布规律,采用多物理场仿真软件COMSOLMultiphysics建立球形细胞五层介电模型,同时引入色散(dispersion,DP)和电穿孔(electroporation,EP)效应来研究纳秒脉冲下的细胞生物电效应。结果表明,在电穿孔的基础上引入色散效应,使得细胞膜上点A1跨膜电位(trans-membranepotential,TMP)达到峰值的速度明显加快,核膜上点B1的跨膜电位在0~100ns区间明显增大;微孔密度、跨膜电位的时空变化结果表明,点A1首先在2ns左右发生电穿孔,随后点A2-A5依次发生电穿孔,最终细胞膜上至少2/3区域发生电穿孔。研究结果从理论上证明了只有同时引入色散和电穿孔效应才能正确预测纳秒脉冲的生物电效应。

In order to accurately study the distribution of cell transmembrane potential under nanosecond pulses electric field(nsPEF), we developed a five-layer dielectric model of spherical cell with multi-physics simulation software of COMSOL Multiphysics, and studied the bioelectric effects of dispersion(DP) and electroporation(EP) on a cell with ns PEF. Compared with the results obtained by only taking into account EP, the speed at which the trans-membrane potential(TMP) of point A1 reaches its peak is obviously accelerated;and significant increase in the TMP of B1 during the period from 0 to 100 ns is observed when both DP and EP are introduced;the pore density and TMPs of points A1-A7 with time and along the arc length show that A1-A5 are electroporated successively with A1 at 2 ns, demonstrating that at least 2/3 of the plasma membrane is electroporated. The simulation results demonstrate that accurate prediction of bioelectric effects of nsPEF can be obtained only introducing both DP and EP.