亚硒酸钠对帕金森模型大鼠运动功能及脑黑质抗氧化能力的影响

Effects of sodium selenite on motor function and antioxidant capacity of substantia nigra in rats with Parkinson's disease

目的:探讨亚硒酸钠对帕金森病(Parkinson's disease,PD)大鼠的运动功能及黑质区抗氧化能力的影响。方法:选取48只爬杆得分为0分的雄性SD大鼠,其中8只作为对照组(Ⅰ组),其余40只连续7 d腹腔注射MPTP建立PD模型。利用行为学筛选建模成功的24只PD大鼠,随机分为MPTP组(Ⅱ组)、MPTP+0.05 mg/kg Se组(Ⅲ组)、MPTP+0.1 mg/kg Se组(Ⅳ组),每组8只。灌胃30 d后对各组行为学、黑质部脂质过氧化物含量、谷胱甘肽过氧化物酶及超氧化物歧化酶的活性、黑质部病理变化、TH +细胞数量及TH mRNA水平进行分析。 结果:与Ⅰ组相比,Ⅱ组的方格间穿行格数显著降低[(95.40±14.66)格,(6.11±4.17)格, P<0.05],竖起次数显著降低,爬杆得分显著增加( P<0.05);MDA含量显著升高[(4.02±0.62)nmol/mg,(12.75±1.59)nmol/mg, P<0.05];SOD、GSH-Px活性显著降低(均 P<0.05);黑质区神经细胞皱缩、神经元数量减少,TH +细胞数和TH mRNA的表达水平显著降低(均 P<0.05) 。与Ⅱ组相比,Ⅲ组与Ⅳ组的方格间穿行格数显著增加[(88.80±24.61)格,(38.86±19.77)格,均 P<0.05],竖起次数显著增加,爬杆得分显著降低(均 P<0.05);Ⅲ组MDA含量显著降低(均 P<0.05);SOD、GSH-Px活性显著升高( P<0.05);黑质区神经细胞结构完整并且排列整齐,TH +细胞数和TH mRNA的表达水平显著增加( P<0.05);Ⅳ组MDA含量没有差异性;SOD、GSH-Px活性升高,但差异无统计学意义( P>0.05);黑质区神经细胞有所恢复,TH +细胞数有所增加,差异无统计学意义( P>0.05)。 结论:0.05 mg/kg的亚硒酸钠能明显改善PD大鼠运动功能,提高黑质部抗氧化能力,从而保护黑质区神经元。

Objective To investigate the effects of sodium selenite on the motor function and antioxidant capacity of substantia nigra in Parkinson's disease(PD)rats.Methods A total of 48 male SD rats with 0 score in pole test were selected,8 of which were used as control group(group I)and the remaining 40 rats were intraperitoneally injected with MPTP for 7 days to establish the PD model.The 24 PD rats with successful modeling were selected out by behavioral analysis,and randomly divided into MPTP group(groupⅡ),MPTP+0.05 mg/kg Se group(groupⅢ),MPTP+0.1 mg/kg Se group(groupⅣ),with 8 rats in each group.After 30 days of gavage,the behaviors of lipid peroxides in the substantia nigra,the activities of glutathione peroxidase and superoxide dismutase,pathological changes in the substantia nigra.The number of TH+cells and TH mRNA levels in each group were analyzed.Results Compared with groupⅠ,the number of crossing of group II was significantly decreased((95.40±14.66),(6.11±4.17),P<0.05),and rearing was significantly decreased and the score of pole test was significantly increased(P<0.05).MDA content was significantly increased((4.02±0.62),(12.75±1.59),P<0.05).The activity of SOD and GSH-Px decreased significantly(P<0.05).The number of neurons in nigra decreased,the number of TH+cells and the expression level of TH mRNA decreased significantly(P<0.05).Compared with group II,group III and group IV showed a significant increase in open field test((88.80±24.61),(38.86±19.77),P<0.05),and a significant decrease in pole test scores(P<0.05).MDA content in groupⅢwas significantly reduced(P<0.05).SOD and GSH-Px activity increased significantly(P<0.05).The nerve cells in the substantia nigra region had complete structure and orderly arrangement,and the number of TH+cells and the expression level of TH mRNA were significantly increased(P<0.05).There was no difference in MDA content in group.SOD and GSH-Px activity increased,but the difference was not significant.The number of TH+cells increased,but the difference was not significant.Conclusion It suggests that 0.05 mg/kg of sodium selenite can significantly improve the motor function of PD rats and enhance the antioxidant capacity of substantia nigra,so as to protect neurons in the substantia nigra.