黄瓜花叶病毒浙江白术分离物全基因组序列测定与分析

Complete genomic sequence analysis of Cucumber mosaic virus isolated from Atractylodes macrocephala Koidz. in Zhejiang

白术(Atractylodes macrocephala Koidz.)为菊科(Compositae)苍术属(Atractylodes)多年生草本植物,其根茎入药具补脾健胃、燥湿利水、止汗安胎等功效,在我国广泛栽培,并以于术(浙江临安于潜)品质最佳[1]。近年来白术生产由于品种单一,长期连作等因素导致病毒病害日趋严重。据报道,黄瓜花叶病毒(Cucumber mosaic virus,CMV)[2]、蚕豆萎蔫病毒2号(Broad bean wilt virus 2.

Virus-infecting leaf samples of Atractylodes macrocephala Koidz. were collected from Lin′an District, Hangzhou city, Zhejiang province. Small RNA(sRNA) library construction and deep sequencing were conducted with samples holding mosaic, chlorosis and dwarf symptoms. The Cucumber mosaic virus(CMV) was specially identified in the samples after assembly of the overall sRNAs. And the complete genomic fragments of CMV(CMV-ZJAm) were verified by PCR and sanger sequencing. The results showed the 3 363 nt fragment of RNA1 sharing 99.01% nucleotide similarity with that of CMV isolate JC(CMV-JC, GenBank accession number MH594044), the 3 053 nt fragment of RNA2 sharing 99.04% similarity(CMV-JC, MH594045), the 2 217 nt RNA3 sharing 97.52% similarity(CMV isolate Anhui, KX660758). Phylogenetic analysis with complete genomic sequences indicated that CMV-ZJAm, CMV-JC, CMV-Am(CMV isolate Am, JX993910) and CMV-Anhui were clustered into one group belonging to CMV subgroup I B. In conclusion, this is the first report of CMV from A. macrocephala in Zhejiang province.