降香黄檀叶枯病菌的鉴定及其生物学特性

Identification and Biological Characteristics of Dalbergia odorifera Leaf Blight

近年来,降香黄檀叶枯病的危害越来越严重,为深入了解该病菌特征,本研究对该病原菌进行鉴定,以期为制定更有效的防治措施提供依据。本研究对降香黄檀叶枯病病株进行病原菌分离,并对病原菌进行了形态学观察及rDNA-ITS序列测定,确定该病原菌为天门冬拟茎点霉菌[Phomopsis asparagi (Sacc.) Bubak]。通过观察该病原菌在不同培养基、温度、pH、光照等4种环境因素中的生长情况,发现该病原菌在马铃薯果糖琼脂培养基(PFA)中菌丝生长最快;菌丝在10~30℃均能生长,最适宜的生长温度为25~30℃;最适宜的pH为6;光照对该病原菌的生长没有显著影响。

In recent years, the harmfulness of Dalbergia odorifera leaf blight has become more and more serious. In order to provide a basis for the control and comprehensive treatment of this disease, the pathogenic fungus causing the disease was isolated and identified and the biological characteristics were studied. In this study, the pathogenic fungus was isolated from the pathogen of D. odorifera leaf blight, and the morphological observation of the pathogenic fungi was carried out. And the rDNA-ITS sequence was obtained by sequencing. The pathogenic fungus was identified as Phomopsis asparagi. Through experiments on the growth medium, temperature, pH value and illumination of the pathogens, the mycelial growth of the fungus in PFA medium was the fastest. The mycelium could grow at 10–30 ℃, and the optimum temperature for mycelium growth was 25-30 ℃. The optimum pH for the mycelium growth was 6. Light had no significant effect on the growth of the pathogen.