西兰花叶中生物活性成分的测定

Determination of Bioactive Ingredients in Broccoli Leaves

为探究西兰花叶中生物活性成分的具体含量,以西兰花叶为原材料,对西兰花叶中萝卜硫素、总酚、总黄酮、总蛋白质的含量和组成进行了测定与分析。结果表明,西兰花废弃叶中萝卜硫素含量为92.50μg·g^-1DW,总酚含量为3.15 mg·g^-1DW,总黄酮含量为2.48 mg·g^-1DW,总蛋白质含量为89.38 mg·g^-1 DW,蛋白质组成中核酮糖-1,5-二磷酸羧化酶/加氧酶(Rubisco)所占比例最大。本研究明确了西兰花叶片中4种生物活性成分的具体含量,为进一步开发利用西兰花废弃物提供了一定的数据支持。

In order to explore the specific content of bioactive ingredients in the broccoli leaves. Broccoli leaves were selected as the research material to analyze the bioactive ingredients contained. The contents of sulforaphane, total phenolics, total flavonoids, total proteins in broccoli leaves were 92.5 μg·g^-1(dry weight), 3.15 mg·g^-1(dry weight), 2.48 mg·g^-1(dry weight) and 89.38 mg·g^-1(dry weight). Ribulose-1,5-diphosphate carboxylase/oxygenase(Rubisco) occupied most of the proteins in broccoli leaves. This study clarified the specific contents of four bioactive components in broccoli leaves, which provided some data support for the further utilization of broccoli waste.