基于LPS诱导RAW 264.7巨噬细胞炎症模型的榴莲壳多酚抗炎作用及其分子机制

Anti-inflammatory effect and molecular mechanism of durian hull polyphenols on LPS-induced RAW 264.7 macrophages

采用80%甲醇溶剂提取榴莲壳中多酚组分,并测定其总酚和总黄酮含量,基于体外化学方法和LPS诱导的RAW 264.7巨噬细胞炎症模型,探究榴莲壳的抗氧化和抗炎活性及其分子机制。结果表明,榴莲壳提取物富含多酚类化合物,具有较强的体外抗氧化活性,且在LPS诱导的RAW 264.7巨噬细胞炎症模型中能降低一氧化氮合酶(iNOS)和环氧合酶-2(COX-2)的表达,减少NO和ROS的产生,并降低炎症细胞因子(TNF-α、IL-1β和IL-6)在基因和蛋白水平上的表达,从而展现较强的抗炎活性。其内在的分子机制可能是通过抑制IκB-α和p65蛋白磷酸化,降低NF-κB信号通路的表达,减少机体的炎症损伤。

In this study,polyphenols from Durian hull were extracted by 80%methanol,and their antioxidant and anti-inflammatory activities were investigated based on in vitro antioxidant assays and LPS-stimulated RAW 264.7 cells model.The results demonstrated that durian hull extract was rich in polyphenols,it showed strong antioxidant activity in vitro and it could reduce the expression level of inducible nitricoxide synthase(iNOS)and cyclooxygenase-2(COX-2),decrease the NO production,and also lower the pro-inflammatory cytokines(TNF-α,IL-1βand IL-6)at the mRNA and protein levels.At the same time,it can inhibit the phosphorylation of IκB and p65 proteins related to the NF-κB signaling pathway and inhibit the expression of NF-κB,thereby reducing inflammatory damages.