摘要
通过选择不同基质(2,5-二羟基苯甲酸、super DHB、2,4,6-三羟基苯乙酮)和设置不同啤酒样品稀释倍数(从原液到1000倍稀释),优化了MALDI TOF MS定性分析啤酒中糖类的试验条件。研究结果显示,3种基质中,2,4,6-三羟基苯乙酮应用于MALDI TOF MS对啤酒中糖类表征的能力优于另外两种基质。当以2,4,6-三羟基苯乙酮为基质,啤酒样品稀释10倍时,MALDI TOF MS可达良好的定性分析效果。
In this study,the experimental condition for qualitative analysis of saccharides in beer was optimized by choosing different matrices,including.2,5-dihydroxybenzoic acid(2,5-DHB),super DHB,2,4,6-trihydroxyacetophenone,and setting different beer concentrations from undiluted to 1000-fold dilution.Results indicated that 2,4,6-trihydroxyacetophenone was better than 2,5-dihydroxybenzoic acid and super DHB in the application of characterizing saccharides in beer by MALDI TOF MS.When the beer sample is diluted 10 times with 2,4,6-trihydroxyacetophenone as matrix,MALDI TOF MS performed well in qualitative analysis of saccharides in beer.
作者
孟贤仁
谢明勇
聂少平
王君巧
MENG Xian-ren;XIE Ming-yong;NIE Shao-ping;WANG jun-qiao(State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang,Jiangxi 330047,China)
出处
《食品与机械》
北大核心
2020年第4期86-90,共5页
Food and Machinery
基金
国家重点研发课题(编号:2017YFC1600405)
江西省重大研发专项(编号:20165ABC28004)
国家自然科学基金青年科学基金项目(编号:31801495)。
