青冈栎果壳提取物体外抗氧化及α-葡萄糖苷酶、乙酰胆碱酯酶抑制能力研究

In vitro antioxidant,α-glucosidase and acetylcholinesterase inhibitory activities of the shell oxtracts from Cyclobalanopsis glauca

以羟基自由基清除能力、2-联苯基-1-苦基肼基(DPPH)自由基清除能力、超氧阴离子清除能力和2,2-联氮-双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)自由基清除能力为指标,评估青冈栎果壳提取物(Cyclobalanopsis glauca Shells Extract,CGSE)的体外抗氧化活性;采用荧光底物法评估CGSE的α-葡萄糖苷酶抑制能力;采用改良的Ellman法评估CGSE的乙酰胆碱酯酶抑制能力。结果表明:CGSE具有较好的抗氧化能力,其清除羟基自由基、DPPH自由基、ABTS自由基和超氧阴离子的IC 50值分别为(273.43±9.55),(8.31±0.08),(26.43±0.14)μg/mL和(1662.37±30.71)μg Trolox/g·DW。CGSE抑制α-葡萄糖苷酶的IC 50值为(24.81±1.99)μg/mL,显著优于对照药物阿卡波糖;CGSE还具有一定的乙酰胆碱酯酶抑制能力,其IC 50值为(422.29±25.26)μg/mL。

The antioxidant activity,hypoglycemic potential,and Alzheimer's therapeutic potential of Cyclobalanopsis glauca Shells were investigated in this study.The hydroxyl radical scavenging ability,DPPH radical scavenging ability,superoxide anion radical scavenging ability,and ABTS radical scavenging ability were used as an indicator for evaluating the in vitro antioxidant activity of Cyclobalanopsis glauca Shells Extract(CGSE).Then theα-glucosidase inhibitory ability of CGSE was evaluated by the fluorimetry assay,and the acetylcholinesterase(AChE)inhibitory ability of CGSE was evaluated by the modified Ellman method.Experimental results showed that CGSE had strong antioxidant activity,their IC 50 values of hydroxyl radical scavenging activity,DPPH radical scavenging activity,ABTS radical scavenging activity,and superoxide anion scavenging activity were(273.43±9.55),(8.31±0.08),(26.43±0.14)μg/mL,and(1662.37±30.71)μg Trolox/g·DW,respectively.The IC 50 values towardα-glucosidase of CGSE was(24.81±1.99)μg/mL,which were significantly higher than that of positive control acarbose.CGSE also showed a certain AChE inhibitory ability with the IC 50 value of(422.29±25.26)μg/mL.