RPN2与肝癌患者预后及对肝癌细胞生长的作用

Prognostic Value of RPN2 in Hepatocellular Carcinoma and Its Effect on the Growth of Hepatocellular Carcinoma Cells

目的:探讨核糖蛋白2(ribophorin II,RPN2)在肝细胞肝癌(HCC)组织中的表达和对HCC患者生存的影响,同时分析RPN2对肝癌HepG2细胞生长和克隆形成的作用。方法:应用免疫组化方法和HCC公共芯片数据,从蛋白和m RNA水平检测HCC组织中RPN2的表达,同时分析RPN2与HCC患者临床参数的关系及预后相关性;进一步利用MTS法和克隆形成实验在肝癌HepG2细胞中检测RPN2对细胞生长的作用。结果:98例肝癌组织中,RPN2阳性表达率88.78%,对应癌旁肝组织中,RPN2阳性表达率74.49%;癌组织中RPN2染色评分为5.80±3.15,癌旁肝组织RPN2染色评分为2.13±1.59,肝癌组织中RPN2表达显著上调(P<0.001)。3个肝癌公共芯片数据(共522例肝癌)中RPN2的m RNA表达水平同样显著升高(均P<0.001)。98例肝癌患者RPN2表达水平与肿瘤直径(P=0.004)、门脉侵袭(P=0.012)和TNM分期(P=0.009)相关;RPN2高表达的患者总体生存期(OS)和无复发生存期(RFS)较RPN2低表达的患者短(OS:P=0.027;RFS:P=0.036)。肝癌HepG2细胞转染RPN2小干扰RNA后,细胞生长能力显著受抑制。结论:RPN2在肝癌中表达显著升高,RPN2的表达与肝癌的恶性进展有关,RPN2显著促进肝癌细胞生长。

Objective: To explore the expression of Ribophorin II(RPN2) in hepatocellular carcinoma(HCC) and its effect on the survival of HCC patients, and to analyze the effects of RPN2 on the growth of HepG2 cells. Methods: The protein and m RNA expression of RPN2 in HCC patients was analyzed by immunohistochemical(IHC) staining and public data of HCC, and to analyze its relationship between the expression of RPN2 and clinicopathological features and prognosis of HCC patients. The effects of RPN2 on the growth of HCC cells were detected by using MTS and cloning formation experiment in HepG2 cells. Results: In 98 HCC tissues, the RPN2 positive expression rate was 88.78%, and the RPN2 positive expression rate was 74.49% in the para-carcinoma tissue. The RPN2 staining score was 5.80±3.15 in HCC tissue, and the RPN2 staining score was 2.13 ±1.59 in the para-carcinoma tissue, the expression of RPN2 in HCC was significantly increased(P<0.001). The expression of RPN2 m RNA in three public data of HCC(522 cases) was also significantly increased(All P<0.001). RPN2 expression was related to tumor diameter(P=0.004), portal vein invasion(P=0.012) and TNM staging(P=0.009) in 98 HCC patients. The overall survival(OS) and recurrence-free survival(RFS) of HCC patients with high expression of RPN2 were shorter than those with RPN2 low expression(OS: P=0.027;RFS: P=0.036). The cell growth was significantly inhibited with the transfection of RPN2 si RNA in HepG2 cells. Conclusion: The expression of RPN2 in HCC was significantly increased,and the expression of RPN2 was related to the progression of HCC. RPN2 promoted HCC cell growth.