核内不均一核糖核蛋白D和上皮细胞黏附分子在口腔鳞状细胞癌组织中的表达及其意义

Expressions of heterogeneous nuclear ribonucleoprotein D and epithelial cell adhesion molecule in oral squamous cell carcinoma tissue and their significances

目的:探讨核内不均一核糖核蛋白D(hnRNPD)和上皮细胞黏附分子(EpCAM)在口腔鳞状细胞癌(OSCC)组织和正常口腔黏膜组织中的表达及其与OSCC患者临床病理特征的关系,阐明其在OSCC发生发展中的作用。方法:选取术前均未经放、化疗的38例OSCC患者的肿瘤组织(OSCC组)和11例正常拔牙患者的口腔黏膜组织(对照组),采用免疫组织化学染色法检测OSCC组织和正常口腔黏膜组织中hnRNPD和EpCAM表达水平,并分析不同临床病理特征OSCC患者肿瘤组织中hnRNPD和EpCAM表达水平,采用Spearman相关性检验分析OSCC组织中hnRNPD和EpCAM表达的相关性。结果:与对照比较,OSCC组肿瘤组织中hnRNPD和EpCAM表达水平升高(Z=-4.936,P=0.000;Z=-2.780,P=0.005);不同性别和年龄OSCC患者肿瘤组织中EpCAM蛋白表达水平差异有统计学意义(Z=-2.471,P=0.013;Z=-1.967;P=0.049);不同临床病理特征OSCC患者肿瘤组织中hnRNPD表达水平比较差异均无统计学意义(P>0.05)。hnRNPD和EpCAM表达水平相关性较弱(ρ=0.227,P=0.17)。结论:OSCC患者肿瘤组织中hnRNPD和EpCAM表达水平上调,两者促进了OSCC的发生发展。

Objective:To investigate the expressions of heterogeneous nuclear ribonucleoprotein D(hnRNPD)and epithelial cell adhesion molecule(EpCAM)in the oral squamous cell carcinoma(OSCC)tissue and normal oral mucosa tissue,and to discuss the associations between their expressions and the clinicopathological parameters of the OSCC patients,and to elucidate their effects on the occurrence and development of OSCC.Methods:The tumor tissue of 38 OSCC patients without preoperative radiotherapy and chemotherapy(OSCC group)and the normal oral mucosa tissue of 11 patients with tooth extraction(control group)were selected.Immunohistochemistry staining was used to detect the expression levels of hnRNPD and EpCAM in the tumor tissue and normal oral mucosa tissue,the expression levels of hnRNPD and EpCAM in tumor tissue of the OSCC patients with different clinicopathological parameters were analyzed,and the correlations between the expression levels of hnRNPD and EpCAM in tumor tissue of the OSCC patients were detected by Spearman correlation test.Results:Compared with control group,the expression levels of hnRNPD and EpCAM in the tumor tissue in OSCC group were increased(Z=-4.936,P=0.000;Z=-2.780,P=0.005);there was statistically significant difference in the expression level of EpCAM in tumor tissue of the OSCC patients with different genders and ages(Z=-2.471,P=0.013;Z=-1.967;P=0.049),there was no significant difference in the expression level of hnRNPD in tumor tissue of the OSCC patients with different clinicopathological parameters(P>0.05).The correlation between the expression levels of hnRNPD and EpCAM was weak(ρ=0.227,P=0.17).Conclusion:The expression levels of hnRNPD and EpCAM in tumor tissue of the OSCC patients are up-regulated,and they promote the occurrence and development of OSCC.