HPLC同时测定不同产地密蒙花中4种化合物的含量

Simultaneous Determination of 4 Components in Buddleja Officinalis Maxim.from Different Regions by HPLC

目的采用HPLC同时测定密蒙花中4种化合物(毛蕊花糖苷、蒙花苷、芹菜素、木犀草素)的含量,并对不同产地的密蒙花进行比较。方法采用Waters Sunfire^TM C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.2%磷酸水溶液为流动相,梯度洗脱;流速1.0 mL·min^-1;柱温30℃;检测波长330 nm(毛蕊花糖苷、蒙花苷、芹菜素)和350 nm(木犀草素)。结果 4种化合物在各自的范围内呈现良好的线性关系(r>0.999 0),平均回收率为97.75%~98.49%,RSD为0.7%~1.5%。测得不同产地毛蕊花糖苷、蒙花苷、芹菜素、木犀草素的含量分别为21.046 3~42.886 3,8.514 4~17.400 8,0.317 3~1.205 3,0.030 4~0.145 6 mg·g^-1。结论该法稳定可靠,重复性好,可为密蒙花质量评价提供一定依据。

OBJECTIVE To establish an HPLC method for simultaneous determination of acteoside,linarin,apigenin,luteolin in Buddleja officinalis Maxim.and to compare the content of Buddleja officinalis Maxim.from different origins.METHODS The analysis was performed on Waters Sunfire^TM C18 analytical column(4.6 mm×250 mm,5μm)with the gradientelution of acetonitrile-0.05%phosphoric acid solution at a flow rate of 1.0 mL·min^-1,the column temperature was 30℃and the detection wavelength was set at 330 nm(acteoside,linarin,apigenin)and 350 nm(luteolin).RESULTS Four constituents showed good linear relationships(r>0.9990)within their own ranges,whose average recoveries were 97.75%-98.49%with the RSDs of 0.7%-1.5%.The contents of acteoside,linarin,apigenin,luteolin in samples of different regions were in ranges of 21.0463-42.8863,8.5144-17.4008,0.3173-1.2053,0.0304-0.1456,respectively.CONCLUSION This reliable,stable and reproducible method can be used to evaluate the quality of Buddleja officinalis Maxim.